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Literature summary for 2.7.1.19 extracted from
- Purification, some catalytic and molecular properties of phosphoribulokinase from Alcaligenes eutrophus (1981), Biochim. Biophys. Acta, 658, 35-44.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ | Cupriavidus necator |  |
| Co2+ | can partially replace Mg2+ | Cupriavidus necator | |
| Mg2+ | required | Cupriavidus necator | |
| Mn2+ | can partially replace Mg2+ | Cupriavidus necator | |
| additional information | not activated by Ni2+ | Cupriavidus necator |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
8 * 33000, subunits of identical size, SDS-PAGE | Cupriavidus necator |
| 252000 | 256000 | sedimentation equilibrium centrifugation, gel filtration | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + ATP | Cupriavidus necator | - |
D-ribulose 1,5-diphosphate + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
basonym Alcaligenes eutrophus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Cupriavidus necator |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Cupriavidus necator |
| 7.59 | - |
- |
Cupriavidus necator |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, no significant loss of activity after 1 month | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 6-phosphate + ATP | 3.6% of ribulose 5-phosphate activity | Cupriavidus necator | ? | - |
? | |
| D-ribulose 5-phosphate + ATP | - |
Cupriavidus necator | D-ribulose 1,5-diphosphate + ADP | - |
? | |
| D-ribulose 5-phosphate + ATP | ATP can be substituted by UTP with reduced activity | Cupriavidus necator | D-ribulose 1,5-diphosphate + ADP | - |
? | |
| D-ribulose 5-phosphate + ATP | ATP can be substituted by GTP with reduced activity | Cupriavidus necator | D-ribulose 1,5-diphosphate + ADP | - |
? | |
| sedoheptulose 7-phosphate + ATP | 2.5% of ribulose 5-phosphate activity | Cupriavidus necator | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 33000, subunits of identical size, SDS-PAGE | Cupriavidus necator |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Cupriavidus necator |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | activation | Cupriavidus necator | |
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Release 2023.1 (January 2023)
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