Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADH | strong activator | Rhodoblastus acidophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphoenolpyruvate | 50% inhibition at 0.32 mM | Rhodoblastus acidophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rhodoblastus acidophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
248000 | - |
sucrose density gradient centrifugation | Rhodoblastus acidophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + ATP | Rhodoblastus acidophilus | - |
D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | Rhodoblastus acidophilus 1150 | - |
D-ribulose 1,5-diphosphate + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodoblastus acidophilus | - |
strain 01150 | - |
Rhodoblastus acidophilus 1150 | - |
strain 01150 | - |
Purification (Comment) | Organism |
---|---|
homogeneity | Rhodoblastus acidophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
111.2 | - |
- |
Rhodoblastus acidophilus |
Storage Stability | Organism |
---|---|
0-4°C, 100 mM imidazole-HCl buffer, pH 7.0, 10 mM MgCl2, 1 mM EDTA, 1 mM dithioerythritol, 3 months | Rhodoblastus acidophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + ATP | - |
Rhodoblastus acidophilus | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by CTP with reduced activity | Rhodoblastus acidophilus | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by UTP with reduced activity | Rhodoblastus acidophilus | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by GTP with reduced activity | Rhodoblastus acidophilus | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by TTP with reduced activity | Rhodoblastus acidophilus | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | - |
Rhodoblastus acidophilus 1150 | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by CTP with reduced activity | Rhodoblastus acidophilus 1150 | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by UTP with reduced activity | Rhodoblastus acidophilus 1150 | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by GTP with reduced activity | Rhodoblastus acidophilus 1150 | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + ATP | ATP can be substituted by TTP with reduced activity | Rhodoblastus acidophilus 1150 | D-ribulose 1,5-diphosphate + ADP | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
presence of NADH | Rhodoblastus acidophilus |
8.4 | - |
absence of NADH | Rhodoblastus acidophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | activation | Rhodoblastus acidophilus | |
NADH | pH-dependent | Rhodoblastus acidophilus |