Cloned (Comment) | Organism |
---|---|
gene lsrK, recombinant expression of N-terminally His-tagged enzyme as C-terminally glutamine-tagged LsrK-Gln and tyrosine-tagged LsrK-Tyr in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | assembly of a capsule of biological polymers alginate and chitosan, with attached/encapsulated AI-2 processing kinase LsrK and provided substrate ATP, for the enzymatic alteration of AI-2 in culture fluids. The functionalized capsules, when introduced into populations of bacteria, alter the dynamics of the AI-2 bacterial communication, attenuating QS activated phenotypes. Method, overview | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (S)-4,5-dihydroxypentane-2,3-dione | Escherichia coli | - |
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P77432 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged LsrK-Gln and LsrK-Tyr from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (S)-4,5-dihydroxypentane-2,3-dione | - |
Escherichia coli | ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62900 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AI-2 kinase | - |
Escherichia coli |
AI-2 processing kinase | - |
Escherichia coli |
lsrK | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | molecular communication among bacteria occurs via quorum sensing (QS). Modulating molecular communication among bacteria can alter phenotype, including attachment to epithelia, biofilm formation, and even toxin production. Intercepting and modulating communication networks provide a means to attenuate virulence without directly interacting with the bacteria of interest | Escherichia coli |
physiological function | communication mediated by the quorum sensing (QS) requires bacterial autoinducer-2, AI-2 ((S)-4,5-dihydroxypentane-2,3-dione). AI-2 mediated QS activity is diminished by phosphorylation via the AI-2 processing kinase, LsrK | Escherichia coli |