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Literature summary for 2.7.1.189 extracted from

  • Rhoads, M.K.; Hauk, P.; Terrell, J.; Tsao, C.Y.; Oh, H.; Raghavan, S.R.; Mansy, S.S.; Payne, G.F.; Bentley, W.E.
    Incorporating LsrK AI-2 quorum quenching capability in a functionalized biopolymer capsule (2018), Biotechnol. Bioeng., 115, 278-289 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene lsrK, recombinant expression of N-terminally His-tagged enzyme as C-terminally glutamine-tagged LsrK-Gln and tyrosine-tagged LsrK-Tyr in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information assembly of a capsule of biological polymers alginate and chitosan, with attached/encapsulated AI-2 processing kinase LsrK and provided substrate ATP, for the enzymatic alteration of AI-2 in culture fluids. The functionalized capsules, when introduced into populations of bacteria, alter the dynamics of the AI-2 bacterial communication, attenuating QS activated phenotypes. Method, overview Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (S)-4,5-dihydroxypentane-2,3-dione Escherichia coli
-
ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P77432
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged LsrK-Gln and LsrK-Tyr from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (S)-4,5-dihydroxypentane-2,3-dione
-
Escherichia coli ADP + (S)-4-hydroxypentane-2,3-dione 5-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 62900 Escherichia coli

Synonyms

Synonyms Comment Organism
AI-2 kinase
-
Escherichia coli
AI-2 processing kinase
-
Escherichia coli
lsrK
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli

General Information

General Information Comment Organism
metabolism molecular communication among bacteria occurs via quorum sensing (QS). Modulating molecular communication among bacteria can alter phenotype, including attachment to epithelia, biofilm formation, and even toxin production. Intercepting and modulating communication networks provide a means to attenuate virulence without directly interacting with the bacteria of interest Escherichia coli
physiological function communication mediated by the quorum sensing (QS) requires bacterial autoinducer-2, AI-2 ((S)-4,5-dihydroxypentane-2,3-dione). AI-2 mediated QS activity is diminished by phosphorylation via the AI-2 processing kinase, LsrK Escherichia coli