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Literature summary for 2.7.1.186 extracted from

  • Vinokur, J.M.; Korman, T.P.; Cao, Z.; Bowie, J.U.
    Evidence of a novel mevalonate pathway in archaea (2014), Biochemistry, 53, 4161-4168.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.186

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 (R)-3-phosphomevalonate pH 8.0, 60°C, it is not possible to accurately determine the Km for mevalonate-3-phosphate-5-kinase, however, because the substrate is not commercially available and the detection limit of the assay is 0.03 mM substrate. The enzyme is still at Vmax when it is assayed with 0.06 mM mevalonate 3-phosphate, indicating that the Km is well below 0.06 mM Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-3-phosphomevalonate Thermoplasma acidophilum mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum ADP + mevalonate 3,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HK44
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-3-phosphomevalonate
-
 Thermoplasma acidophilum ADP + mevalonate 3,5-bisphosphate
-
 ?
ATP + (R)-3-phosphomevalonate mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum ADP + mevalonate 3,5-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
Ta0762 locus name Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, enzyme retains more than 95% of its activity Thermoplasma acidophilum
70
-
 1 h, about 75% loss of activity Thermoplasma acidophilum
80
-
 1 h, complete loss of activity Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
 (R)-3-phosphomevalonate pH 8.0, 60°C Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9 no significant pH dependence in the pH range of 6.5-9.0 Thermoplasma acidophilum
8
-
 assay at Thermoplasma acidophilum

General Information

General Information Comment Organism
physiological function mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase act sequentially in a putative alternate mevalonate pathway in Thermoplasma acidophilum Thermoplasma acidophilum