Cloned (Comment) | Organism |
---|---|
recombinant expression of the PtM3K gene under a J23105 promoter | Picrophilus torridus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Picrophilus torridus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-mevalonate | Picrophilus torridus | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | Picrophilus torridus ATCC 700027 | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | Picrophilus torridus NBRC 100828 | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | Picrophilus torridus DSM 9790 | - |
ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | Picrophilus torridus JCM 10055 | - |
ADP + (R)-3-phosphomevalonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | Q6KZB1 | - |
- |
Picrophilus torridus ATCC 700027 | Q6KZB1 | - |
- |
Picrophilus torridus DSM 9790 | Q6KZB1 | - |
- |
Picrophilus torridus JCM 10055 | Q6KZB1 | - |
- |
Picrophilus torridus NBRC 100828 | Q6KZB1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus ATCC 700027 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus NBRC 100828 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus DSM 9790 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
ATP + (R)-2,3-dihydroxyisovalerate | the Picrophilus torridus mevalonate-3-kinase (M3K) exhibits adenosine triphosphate (ATP) hydrolysis activity when mixed with (R)-2,3-dihydroxyisovalerate (DHIV), indicating phosphorylation activity towards DHIV | Picrophilus torridus JCM 10055 | ADP + (R)-2-hydroxy-3-phosphoisovalerate | - |
? | |
ATP + (R)-mevalonate | - |
Picrophilus torridus | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | - |
Picrophilus torridus ATCC 700027 | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | - |
Picrophilus torridus NBRC 100828 | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | - |
Picrophilus torridus DSM 9790 | ADP + (R)-3-phosphomevalonate | - |
? | |
ATP + (R)-mevalonate | - |
Picrophilus torridus JCM 10055 | ADP + (R)-3-phosphomevalonate | - |
? | |
additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus | ? | - |
- |
|
additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus ATCC 700027 | ? | - |
- |
|
additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus NBRC 100828 | ? | - |
- |
|
additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus DSM 9790 | ? | - |
- |
|
additional information | a first reaction strategy is the activaion of (R)-2,3-dihydroxyisovalerate (DHIV) to 3-phospho-DHIV using M3K followed by dehydration by PpManR, StPutD or other sugar acid dehydratases. A second strategy is to use M3K and/or mevalonate decarboxylase (MVD) to convert DHIV to isobutyraldehyde, which can be an alternative pathway for isobutanol biosynthesis | Picrophilus torridus JCM 10055 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
M3K | - |
Picrophilus torridus |
mevalonate-3-kinase | - |
Picrophilus torridus |
PtM3K | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Picrophilus torridus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is specialized as a mevalonate 3-kinase catalyzing the first step of the mevalonate decarboxylation (MVD) reaction | Picrophilus torridus |