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Literature summary for 2.7.1.183 extracted from

  • Xu, C.; Zhang, M.; Bian, L.; Li, Y.; Yao, Y.; Li, D.
    N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells (2020), Oncogenesis, 9, 4 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.183

Cloned(Commentary)

Cloned (Comment) Organism
gene SGK196 or POMK, located on chromosome 8p11, overexpression of SGK196-wild-type and SGK196-4NQ in MDA-MB-231 and BT549, respectively Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information generation of the SGK196-4NQ mutant with several N to Q amino acid exchanges. The PNGase F or Endo H treatment assay also indicates that SGK196-4NQ mutant is totally deglycosylated. The NQ mutations do not affect the stability or the subcellular localization of SGK196 protein in both MDA-MB-231 and BT-549 cells. SGK196 depletion in both MDA-MB-231 and BT-549 cells significantly increases cell migration and invasion. Re-supply of wild-type SGK196 in MDA-MB-231shSGK196 cell line significantly decreases the number of lung metastasis nodules, when compared with the empty vector, by inhibiting the PI3K/AKT/GSK3beta signaling pathway Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane type II transmembrane protein Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-seryl-[protein] Homo sapiens
-
 ADP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-6-O-phosphono-alpha-D-mannosyl]-L-seryl-[protein]
-
 ?
ATP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein] Homo sapiens
-
 ADP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-6-O-phosphono-alpha-D-mannosyl]-L-threonyl-[protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9H5K3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein SGK196 is primarily modified by N-glycosylation in breast cancer cells. N-glycosylation of SGK196 leads to suppression of cell migration, invasion, and metastasis in breast cancer cells, particularly in the basal-like breast cancer (BLBC) type. In addition, enzyme SGK196 N-glycosylation performs the regulatory function through the PI3K/AKT/GSK3beta signaling pathway. N-glycosylated SGK196 plays suppression roles in BLBC metastases. The phosphorylation of the enzyme can be abolished by RPN1 knockout Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Homo sapiens
-
breast cancer cell
-
 Homo sapiens
-
BT-549 cell
-
 Homo sapiens
-
heart
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
lung cancer cell
-
 Homo sapiens
-
MDA-MB-231 cell
-
 Homo sapiens
-
skeletal muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-seryl-[protein]
-
 Homo sapiens ADP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-6-O-phosphono-alpha-D-mannosyl]-L-seryl-[protein]
-
 ?
ATP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-alpha-D-mannosyl]-L-threonyl-[protein]
-
 Homo sapiens ADP + 3-O-[N-acetyl-beta-D-galactosaminyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-6-O-phosphono-alpha-D-mannosyl]-L-threonyl-[protein]
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Homo sapiens
More as a type II transmembrane protein, SGK196 consists of a cytoplasmic tail, one transmembrane helix, and a luminal domain. Lack of functional motifs of protein kinases including Asp-Phe-Gly (DFG), His-Arg-Asp (HRD) and conserved Lys residues in the primary structure of SGK196 Homo sapiens

Synonyms

Synonyms Comment Organism
POMK
-
 Homo sapiens
protein kinase-like protein SgK196 UniProt Homo sapiens
protein O-mannose kinase
-
 Homo sapiens
SGK196
-
 Homo sapiens
sugen kinase 196 UniProt Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function SGK196 is a protein O-mannose kinase involved in an indispensable phosphorylation step during laminin-binding glycan synthesis on alpha-dystroglycan (alpha-DG). SGK196 functions as a glycosylationspecific kinase. SGK196 is involved in the phosphorylation of M3 glycan mannose on the C-6 position within the M3 O-glycan of alpha-dystroglycan (alpha-DG). alpha-DG is a peripheral membrane protein, serving as a receptor for various extracellular matrix components, such as laminin, agrin, perlecan. Extensive glycosylation of alpha-DG is essential for its binding to extracellular matrix ligands. N-glycosylated SGK196 suppresses cell migration, invasion, and metastasis in breast cancer cells, particularly in the basal-like breast cancer (BLBC) type. In addition, enzyme SGK196 N-glycosylation performs the regulatory function through the PI3K/AKT/GSK3beta signaling pathway. N-glycosylated SGK196 plays suppression roles in BLBC metastases, SGK196 N-glycosylation is required for repression of metastasis Homo sapiens