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Literature summary for 2.7.1.182 extracted from

  • Vom Dorp, K.; Hoelzl, G.; Plohmann, C.; Eisenhut, M.; Abraham, M.; Weber, A.P.; Hanson, A.D.; Doermann, P.
    Remobilization of phytol from chlorophyll degradation is essential for tocopherol synthesis and growth of Arabidopsis (2015), Plant Cell, 27, 2846-2859.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene VTE5, recombinant functional expression of gene VTE5 or functional coexpression of genes VTE5 and VTE6 in Escherichia coli, quantitative real-time PCR expression analysis Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of an independent mutant allele (pst12490, vte5-2) that carries a transposon insertion in the first exon behind the start codon of the VTE5 gene and shows no vte5 expression and absence of VTE5 mRNA. Construction of VTE5/VTE6 double mutant plants, which are tocopherol-deficient and contain more chlorophyll, but reduced amounts of phytol and phytyl-phosphate compared with vte6 mutants, suggesting that phytol or phytyl-phosphate are detrimental to plant growth. The growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type, the different forms of fatty acid phytyl esters are increased in leaves of vte5-2, vte6-1, vte6-2, and vte5-2 vte6-1 compared with the wild type, especially 12:0-phytol, 14:0-phytol, and 16:0-phytol Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + phytol Arabidopsis thaliana
-
CDP + phytyl phosphate phytyl phosphate is phosphorylated by phytyl phosphate kinase, VTE6 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LZ76 gene VTE5 or At5g04490
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
seed
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + phytol
-
Arabidopsis thaliana CDP + phytyl phosphate phytyl phosphate is phosphorylated by phytyl phosphate kinase, VTE6 ?

Synonyms

Synonyms Comment Organism
VTE5
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
CTP
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction the growth defect of vte6-1 is alleviated in the vte5-2/vte6-1 double mutant. The tocopherol-deficient mutants vte1, vte2, vte4, and vte5 can grow on soil and produce fertile seeds, while vte6-1 and vte6-2 cannot grow photoautotrophically and are infertile. Amounts of phytol are unchanged in vte5-2 and vte5-2/vte6-1 mutants compared with the wild-type Arabidopsis thaliana
metabolism phytol from chlorophyll degradation can be phosphorylated to phytyl-phosphate by phytol kinase, EC 2.7.1.182, and to phytyl-diphosphate, the substrate for tocopherol (vitamin E) synthesis by phytyl-diphosphate kinase, EC 2.7.4.BX. Tocopherol synthesis in leaves depends on phytol derived from chlorophyll, not on de novo synthesis of phytyl-diphosphate from geranylgeranyl-diphosphate. The phytol phosphorylation pathway is essential for plant growth and development Arabidopsis thaliana
physiological function the essential enzyme is required for tocopherol synthesis and photoautotrophic growth synthesizing phytyl phosphate from phytol from degenerated chlorophyll in the phytol phosphorylation pathway Arabidopsis thaliana