Literature summary for 2.7.1.176 extracted from

Ng, S.L.; Harikrishna, J.A.; Bakar, F.A.; Yeo, C-C.; Cha, T.S.
Heterologous expression of the Streptococcus pneumoniae yoeB and pezT toxin genes is lethal in Chlorella vulgaris (2016), Algal Res., 19, 21-29.

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Application

Application	Comment	Organism
biotechnology	lethal expression of bacterial toxin-antitoxin system toxins in eukaryotic microalgae, which can form the basis of a novel method for harvesting of microalgal cellular contents. Chlorella vulgaris is a eukaryotic microalga with potential for the production of biofuels. Its thick and rigid cell wall is an impediment to cost-effective, large-scale harvesting of biofuels from these cells	Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
gene pezT, recombinant expression of GFP-tagged enzyme in the eukaryotic microalga Chlorella vulgaris strain UMT-M1, Agrobacterium tumefaciens strain LBA4404-mediated co-transformation with recombinant activator and responder vectors, is lethal. Following induction by 17-beta-estradiol for the expression of the toxin-GFP fusion transgenes, algal cells show signs of cellular damage and lysis. Vector construct overview	Streptococcus pneumoniae

Cloned (Comment)

Organism

gene pezT, recombinant expression of GFP-tagged enzyme in the eukaryotic microalga Chlorella vulgaris strain UMT-M1, Agrobacterium tumefaciens strain LBA4404-mediated co-transformation with recombinant activator and responder vectors, is lethal. Following induction by 17-beta-estradiol for the expression of the toxin-GFP fusion transgenes, algal cells show signs of cellular damage and lysis. Vector construct overview

Streptococcus pneumoniae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + UDP-N-acetyl-alpha-D-glucosamine	Streptococcus pneumoniae	-	ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	Q97QZ1	gene pezT	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + UDP-N-acetyl-alpha-D-glucosamine	-	Streptococcus pneumoniae	ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
PezT	-	Streptococcus pneumoniae
toxin PezT	UniProt	Streptococcus pneumoniae
UNAG kinase	-	Streptococcus pneumoniae
Zeta-toxin	UniProt	Streptococcus pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Streptococcus pneumoniae