BRENDA - Enzyme Database
show all sequences of 2.7.1.173

Novel assay for simultaneous measurement of pyridine mononucleotides synthesizing activities allows dissection of the NAD+ biosynthetic machinery in mammalian cells

Zamporlini, F.; Ruggieri, S.; Mazzola, F.; Amici, A.; Orsomando, G.; Raffaelli, N.; FEBS J. 281, 5104-5119 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
diagnostics
screening of tumors for NRK activity is necessary for the design of efficient NamPRT-targeted antitumor therapy
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
heart
-
Homo sapiens
-
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
lung
-
Homo sapiens
-
additional information
ubiquitous expression of nicotinamide riboside kinase in tissues and cell lines
Homo sapiens
-
skeletal muscle
-
Homo sapiens
-
spleen
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + beta-D-ribosylnicotinate
-
738213
Homo sapiens
ADP + nicotinate beta-D-ribonucleotide
-
-
-
?
additional information
development of a highly sensitive, rapid and specific coupled enzymatic assay that allows simultaneous determination of NRK activitiy, as well as of nicotinic acid phosphoribosyltransferase, quinolinic acid phosphoribosyltransferase, and nicotinamide phosphoribosyltransferase activities, in various biological samples of various origins, evaluation and optimization using liver extracts, overview
738213
Homo sapiens
?
-
-
-
-
Application (protein specific)
Application
Commentary
Organism
diagnostics
screening of tumors for NRK activity is necessary for the design of efficient NamPRT-targeted antitumor therapy
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Homo sapiens
-
heart
-
Homo sapiens
-
kidney
-
Homo sapiens
-
liver
-
Homo sapiens
-
lung
-
Homo sapiens
-
additional information
ubiquitous expression of nicotinamide riboside kinase in tissues and cell lines
Homo sapiens
-
skeletal muscle
-
Homo sapiens
-
spleen
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + beta-D-ribosylnicotinate
-
738213
Homo sapiens
ADP + nicotinate beta-D-ribonucleotide
-
-
-
?
additional information
development of a highly sensitive, rapid and specific coupled enzymatic assay that allows simultaneous determination of NRK activitiy, as well as of nicotinic acid phosphoribosyltransferase, quinolinic acid phosphoribosyltransferase, and nicotinamide phosphoribosyltransferase activities, in various biological samples of various origins, evaluation and optimization using liver extracts, overview
738213
Homo sapiens
?
-
-
-
-
General Information
General Information
Commentary
Organism
metabolism
distinct metabolic routes, starting from various precursors, are known to support NAD+ biosynthesis with tissue/cell-specific efficiencies, probably reflecting differential expression of the corresponding rate-limiting enzymes, i.e. nicotinamide phosphoribosyltransferase, quinolinate phosphoribosyltransferase, nicotinate phosphoribosyltransferase and nicotinamide riboside kinase
Homo sapiens
physiological function
nicotinamide riboside is a relevant NAD+ precursor
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
metabolism
distinct metabolic routes, starting from various precursors, are known to support NAD+ biosynthesis with tissue/cell-specific efficiencies, probably reflecting differential expression of the corresponding rate-limiting enzymes, i.e. nicotinamide phosphoribosyltransferase, quinolinate phosphoribosyltransferase, nicotinate phosphoribosyltransferase and nicotinamide riboside kinase
Homo sapiens
physiological function
nicotinamide riboside is a relevant NAD+ precursor
Homo sapiens
Other publictions for EC 2.7.1.173
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739212
Sociali
Antitumor effect of combined N ...
Homo sapiens
Oncotarget
7
2968-2984
2016
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1
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1
1
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738213
Zamporlini
Novel assay for simultaneous m ...
Homo sapiens
FEBS J.
281
5104-5119
2014
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8
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2
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2
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698901
Belenky
Nicotinamide riboside and nico ...
Saccharomyces cerevisiae
J. Biol. Chem.
284
158-164
2009
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3
3
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718225
Matsui
Nicotinate riboside salvage in ...
Vigna radiata var. radiata
Plant Physiol. Biochem.
46
104-108
2008
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1
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1
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1
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3
1
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1
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1
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1
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1
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3
1
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1
1
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682879
Khan
Crystal structure of human nic ...
Homo sapiens
Structure
15
1005-1013
2007
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1
1
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1
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1
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1
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1
1
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4
1
1
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1
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1
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1
1
1
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1
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1
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4
1
1
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1
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1
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718250
Tempel
Nicotinamide riboside kinase s ...
Homo sapiens
PLoS Biol.
5
e263
2007
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1
1
5
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12
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1
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4
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2
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1
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18
1
1
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12
1
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3
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2
4
1
5
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12
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2
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4
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2
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18
2
2
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12
2
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2
4
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12
12