BRENDA - Enzyme Database
show all sequences of 2.7.1.173

Nicotinamide riboside kinase structures reveal new pathways to NAD+

Tempel, W.; Rabeh, W.M.; Bogan, K.L.; Belenky, P.; Wojcik, M.; Seidle, H.F.; Nedyalkova, L.; Yang, T.; Sauve, A.A.; Park, H.W.; Brenner, C.; PLoS Biol. 5, e263 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
His-tagged wild-type Nrk1 expression in Escherichia coli, expression of wild-type and mutant Nrk1 from the GAL1 promoter on a LEU2 plasmid in Saccharomyces cerevisiae strain BY278; His-tagged wild-type Nrk2 expression in Escherichia coli, expression of wild-type and mutant Nrk2 from the GAL1 promoter on a LEU2 plasmid in Saccharomyces cerevisiae strain BY278
Homo sapiens
Crystallization (Commentary)
Crystallization
Organism
Nrk1 bound to nucleoside and nucleotide substrates and products, i.e. nicotinamide mononucleotide, ADP, tiazofurin, beta-D-ribosylnicotinate with AppNHp, and beta-D-ribosylnicotinate alone, addition of 10 mM ligand and 20 mM MgCl2, overview. Usage of different crystallization solutions depending on the ligand, containing PEG 2000 mono-methylether or PEG 3350 or PEG 4000 at 15-35%, at pH 5.0-8.0, with 0.2 M NaH2PO4, and different buffers, X-ray diffraction structure determination and analysis at 1.32-1.95 A resolution, molecular replacement
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
D35A
site-directed mutagenesis of Nrk2
Homo sapiens
D36A
site-directed mutagenesis of Nrk1
Homo sapiens
E100A
site-directed mutagenesis of Nrk2, mutant activity in ATP-dependent phosphorylation is below the level of detection
Homo sapiens
E174R
site-directed mutagenesis of Nrk2
Homo sapiens
E98A
site-directed mutagenesis of Nrk1, mutant activity in ATP-dependent phosphorylation is below the level of detection
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.051
-
beta-D-ribosylnicotinamide
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.063
-
beta-D-ribosylnicotinamide
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.068
-
beta-D-ribosylnicotinate
Nrk1, with GTP, pH 7.5, 37C
Homo sapiens
0.088
-
beta-D-ribosylnicotinate
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.11
-
tiazofurin
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.19
-
beta-D-ribosylnicotinate
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.27
-
tiazofurin
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
1.3
-
uridine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
15
-
cytidine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
17
-
uridine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
30
-
beta-D-ribosylnicotinate
Nrk2, with GTP, pH 7.5, 37C
Homo sapiens
30
-
cytidine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required; required
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + beta-D-ribosylnicotinate
Homo sapiens
nicotinic acid riboside is a specific substrate of human Nrk enzymes
ADP + nicotinate beta-D-ribonucleotide
-
-
?
additional information
Homo sapiens
Asp35 and Glu100 in Nrk2 are essential residues for function in vivo
?
-
-
-
additional information
Homo sapiens
Asp36 and Glu98 in Nrk1 are essential residues for function in vivo
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9NPI5
Nrk2; Nrk2
-
Homo sapiens
Q9NWW6
Nrk1; Nrk1
-
Purification (Commentary)
Commentary
Organism
recombinant Nrk1 and Nrk2 from Escherichia coli by metal chelate affinity chromatography; recombinant Nrk2 from Escherichia coli by metal chelate affinity chromatography
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + beta-D-ribosylnicotinamide
beta-D-ribosylnicotinamide is an NAD+ precursor utilized via the Nrk and Preiss-Hander pathways
718250
Homo sapiens
ADP + nicotinamide beta-D-ribonucleotide
-
-
-
?
ATP + beta-D-ribosylnicotinate
nicotinic acid riboside is a specific substrate of human Nrk enzymes
718250
Homo sapiens
ADP + nicotinate beta-D-ribonucleotide
-
-
-
?
ATP + cytidine
-
718250
Homo sapiens
ADP + CMP
-
-
-
?
ATP + cytidine
low activity with Nrk1
718250
Homo sapiens
ADP + CMP
-
-
-
?
ATP + nicotinamide mononucleotide
-
718250
Homo sapiens
ADP + nicotinamide mononucleotide phosphate
-
-
-
?
ATP + tiazofurin
-
718250
Homo sapiens
ADP + ?
-
-
-
?
ATP + tiazofurin
a relatively good Nrk1 substrate
718250
Homo sapiens
ADP + ?
-
-
-
?
ATP + uridine
-
718250
Homo sapiens
ADP + UMP
-
-
-
?
ATP + uridine
low activity with Nrk1
718250
Homo sapiens
ADP + UMP
-
-
-
?
GTP + beta-D-ribosylnicotinate
nicotinic acid riboside is a specific substrate of human Nrk enzymes
718250
Homo sapiens
GDP + nicotinate beta-D-ribonucleotide
-
-
-
?
additional information
Asp35 and Glu100 in Nrk2 are essential residues for function in vivo
718250
Homo sapiens
?
-
-
-
-
additional information
Asp36 and Glu98 in Nrk1 are essential residues for function in vivo
718250
Homo sapiens
?
-
-
-
-
additional information
substrate specificity of recombinant Nrk1 and Nrk2, overview. Classification of Nrk1 as an nicotinic acid riboside and tiazofurin:ATP or GTP kinase, Nrk1 displays a 340fold preference for nicotinic acid riboside over cytidine in the KM term and a 500fold preference over either cytidine or uridine in the kcat/KM term. Poor cytidine monophosphate-forming activity of the enzyme. Structural basis of substrate specificity, overview. Base recognition in the Nrk1 nucleoside-binding site excludes uridine but supports beta-D-ribosylnicotinamide phosphorylation
718250
Homo sapiens
?
-
-
-
-
additional information
substrate specificity of recombinant Nrk2, overview. Classification of Nrk2 as an ATP-specific nicotinic acid riboside, tiazofurin, and uridine kinase. Poor cytidine monophosphate-forming activity of the enzyme. Structural basis of substrate specificity, overview
718250
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
the ADP-binding site includes P-loop sequence Gly-Val-Thr-Asn-Ser-Gly-Lys-Thr, residues 10-17; the ADP-binding site includes P-loop sequence Gly-Val-Thr-Asn-Ser-Gly-Lys-Thr, residues 10-17
Homo sapiens
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at; assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
beta-D-ribosylnicotinamide
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.21
-
uridine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.34
-
beta-D-ribosylnicotinamide
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.34
-
beta-D-ribosylnicotinate
Nrk1, with GTP, pH 7.5, 37C
Homo sapiens
0.35
-
tiazofurin
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.48
-
cytidine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.49
-
tiazofurin
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.6
-
beta-D-ribosylnicotinate
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.75
-
beta-D-ribosylnicotinate
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.82
-
cytidine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
1.1
-
uridine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
1.7
-
beta-D-ribosylnicotinate
Nrk2, with GTP, pH 7.5, 37C
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at; assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
active with Nrk1; specific for Nrk2
Homo sapiens
GTP
active with Nrk1
Homo sapiens
additional information
GTP is not with Nrk2
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged wild-type Nrk1 expression in Escherichia coli, expression of wild-type and mutant Nrk1 from the GAL1 promoter on a LEU2 plasmid in Saccharomyces cerevisiae strain BY278
Homo sapiens
His-tagged wild-type Nrk2 expression in Escherichia coli, expression of wild-type and mutant Nrk2 from the GAL1 promoter on a LEU2 plasmid in Saccharomyces cerevisiae strain BY278
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
active with Nrk1
Homo sapiens
ATP
specific for Nrk2
Homo sapiens
GTP
active with Nrk1
Homo sapiens
additional information
GTP is not with Nrk2
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
Nrk1 bound to nucleoside and nucleotide substrates and products, i.e. nicotinamide mononucleotide, ADP, tiazofurin, beta-D-ribosylnicotinate with AppNHp, and beta-D-ribosylnicotinate alone, addition of 10 mM ligand and 20 mM MgCl2, overview. Usage of different crystallization solutions depending on the ligand, containing PEG 2000 mono-methylether or PEG 3350 or PEG 4000 at 15-35%, at pH 5.0-8.0, with 0.2 M NaH2PO4, and different buffers, X-ray diffraction structure determination and analysis at 1.32-1.95 A resolution, molecular replacement
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D35A
site-directed mutagenesis of Nrk2
Homo sapiens
D36A
site-directed mutagenesis of Nrk1
Homo sapiens
E100A
site-directed mutagenesis of Nrk2, mutant activity in ATP-dependent phosphorylation is below the level of detection
Homo sapiens
E174R
site-directed mutagenesis of Nrk2
Homo sapiens
E98A
site-directed mutagenesis of Nrk1, mutant activity in ATP-dependent phosphorylation is below the level of detection
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.051
-
beta-D-ribosylnicotinamide
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.063
-
beta-D-ribosylnicotinamide
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.068
-
beta-D-ribosylnicotinate
Nrk1, with GTP, pH 7.5, 37C
Homo sapiens
0.088
-
beta-D-ribosylnicotinate
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.11
-
tiazofurin
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.19
-
beta-D-ribosylnicotinate
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.27
-
tiazofurin
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
1.3
-
uridine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
15
-
cytidine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
17
-
uridine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
30
-
beta-D-ribosylnicotinate
Nrk2, with GTP, pH 7.5, 37C
Homo sapiens
30
-
cytidine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + beta-D-ribosylnicotinate
Homo sapiens
nicotinic acid riboside is a specific substrate of human Nrk enzymes
ADP + nicotinate beta-D-ribonucleotide
-
-
?
additional information
Homo sapiens
Asp35 and Glu100 in Nrk2 are essential residues for function in vivo
?
-
-
-
additional information
Homo sapiens
Asp36 and Glu98 in Nrk1 are essential residues for function in vivo
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant Nrk1 and Nrk2 from Escherichia coli by metal chelate affinity chromatography
Homo sapiens
recombinant Nrk2 from Escherichia coli by metal chelate affinity chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + beta-D-ribosylnicotinamide
beta-D-ribosylnicotinamide is an NAD+ precursor utilized via the Nrk and Preiss-Hander pathways
718250
Homo sapiens
ADP + nicotinamide beta-D-ribonucleotide
-
-
-
?
ATP + beta-D-ribosylnicotinate
nicotinic acid riboside is a specific substrate of human Nrk enzymes
718250
Homo sapiens
ADP + nicotinate beta-D-ribonucleotide
-
-
-
?
ATP + cytidine
-
718250
Homo sapiens
ADP + CMP
-
-
-
?
ATP + cytidine
low activity with Nrk1
718250
Homo sapiens
ADP + CMP
-
-
-
?
ATP + nicotinamide mononucleotide
-
718250
Homo sapiens
ADP + nicotinamide mononucleotide phosphate
-
-
-
?
ATP + tiazofurin
-
718250
Homo sapiens
ADP + ?
-
-
-
?
ATP + tiazofurin
a relatively good Nrk1 substrate
718250
Homo sapiens
ADP + ?
-
-
-
?
ATP + uridine
-
718250
Homo sapiens
ADP + UMP
-
-
-
?
ATP + uridine
low activity with Nrk1
718250
Homo sapiens
ADP + UMP
-
-
-
?
GTP + beta-D-ribosylnicotinate
nicotinic acid riboside is a specific substrate of human Nrk enzymes
718250
Homo sapiens
GDP + nicotinate beta-D-ribonucleotide
-
-
-
?
additional information
Asp35 and Glu100 in Nrk2 are essential residues for function in vivo
718250
Homo sapiens
?
-
-
-
-
additional information
Asp36 and Glu98 in Nrk1 are essential residues for function in vivo
718250
Homo sapiens
?
-
-
-
-
additional information
substrate specificity of recombinant Nrk1 and Nrk2, overview. Classification of Nrk1 as an nicotinic acid riboside and tiazofurin:ATP or GTP kinase, Nrk1 displays a 340fold preference for nicotinic acid riboside over cytidine in the KM term and a 500fold preference over either cytidine or uridine in the kcat/KM term. Poor cytidine monophosphate-forming activity of the enzyme. Structural basis of substrate specificity, overview. Base recognition in the Nrk1 nucleoside-binding site excludes uridine but supports beta-D-ribosylnicotinamide phosphorylation
718250
Homo sapiens
?
-
-
-
-
additional information
substrate specificity of recombinant Nrk2, overview. Classification of Nrk2 as an ATP-specific nicotinic acid riboside, tiazofurin, and uridine kinase. Poor cytidine monophosphate-forming activity of the enzyme. Structural basis of substrate specificity, overview
718250
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
the ADP-binding site includes P-loop sequence Gly-Val-Thr-Asn-Ser-Gly-Lys-Thr, residues 10-17
Homo sapiens
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
beta-D-ribosylnicotinamide
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.21
-
uridine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.34
-
beta-D-ribosylnicotinamide
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.34
-
beta-D-ribosylnicotinate
Nrk1, with GTP, pH 7.5, 37C
Homo sapiens
0.35
-
tiazofurin
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.48
-
cytidine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.49
-
tiazofurin
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.6
-
beta-D-ribosylnicotinate
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.75
-
beta-D-ribosylnicotinate
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.82
-
cytidine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
1.1
-
uridine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
1.7
-
beta-D-ribosylnicotinate
Nrk2, with GTP, pH 7.5, 37C
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
evolution
the enzyme is structurally similar to Rossmann fold metabolite kinases; the enzyme is structurally similar to Rossmann fold metabolite kinases
Homo sapiens
metabolism
the enzyme is involved in the eukaryotic nicotinamide riboside kinase, Nrk, pathway, which is induced in response to nerve damage and promotes replicative life span in yeast, converts nicotinamide riboside to NAD+ by phosphorylation and adenylylation, overview. Nicotinic acid riboside is utilized in vivo by Urh1, Pnp1, and Preiss-Handler salvage; the enzyme is involved in the eukaryotic nicotinamide riboside kinase, Nrk, pathway, which is induced in response to nerve damage and promotes replicative life span in yeast, converts nicotinamide riboside to NAD+ by phosphorylation and adenylylation, overview. Nicotinic acid riboside is utilized in vivo by Urh1, Pnp1, and Preiss-Handler salvage
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme is structurally similar to Rossmann fold metabolite kinases
Homo sapiens
metabolism
the enzyme is involved in the eukaryotic nicotinamide riboside kinase, Nrk, pathway, which is induced in response to nerve damage and promotes replicative life span in yeast, converts nicotinamide riboside to NAD+ by phosphorylation and adenylylation, overview. Nicotinic acid riboside is utilized in vivo by Urh1, Pnp1, and Preiss-Handler salvage
Homo sapiens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.012
-
uridine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.016
-
cytidine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.055
-
cytidine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.057
-
beta-D-ribosylnicotinate
Nrk2, with GTP, pH 7.5, 37C
Homo sapiens
0.85
-
uridine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
1.3
-
tiazofurin
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
3.9
-
beta-D-ribosylnicotinate
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
4.1
-
beta-D-ribosylnicotinamide
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
4.5
-
tiazofurin
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
5
-
beta-D-ribosylnicotinate
Nrk1, with GTP, pH 7.5, 37C
Homo sapiens
5.4
-
beta-D-ribosylnicotinamide
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
6.8
-
beta-D-ribosylnicotinate
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.012
-
uridine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.016
-
cytidine
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
0.055
-
cytidine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
0.057
-
beta-D-ribosylnicotinate
Nrk2, with GTP, pH 7.5, 37C
Homo sapiens
0.85
-
uridine
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
1.3
-
tiazofurin
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
3.9
-
beta-D-ribosylnicotinate
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
4.1
-
beta-D-ribosylnicotinamide
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
4.5
-
tiazofurin
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
5
-
beta-D-ribosylnicotinate
Nrk1, with GTP, pH 7.5, 37C
Homo sapiens
5.4
-
beta-D-ribosylnicotinamide
Nrk2, with ATP, pH 7.5, 37C
Homo sapiens
6.8
-
beta-D-ribosylnicotinate
Nrk1, with ATP, pH 7.5, 37C
Homo sapiens
Other publictions for EC 2.7.1.173
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739212
Sociali
Antitumor effect of combined N ...
Homo sapiens
Oncotarget
7
2968-2984
2016
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
738213
Zamporlini
Novel assay for simultaneous m ...
Homo sapiens
FEBS J.
281
5104-5119
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
8
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
698901
Belenky
Nicotinamide riboside and nico ...
Saccharomyces cerevisiae
J. Biol. Chem.
284
158-164
2009
-
-
-
-
-
-
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
718225
Matsui
Nicotinate riboside salvage in ...
Vigna radiata var. radiata
Plant Physiol. Biochem.
46
104-108
2008
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
3
1
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
3
1
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
682879
Khan
Crystal structure of human nic ...
Homo sapiens
Structure
15
1005-1013
2007
-
-
1
1
-
-
-
-
-
1
-
1
-
1
-
-
1
1
-
-
-
-
4
1
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
718250
Tempel
Nicotinamide riboside kinase s ...
Homo sapiens
PLoS Biol.
5
e263
2007
-
-
1
1
5
-
-
12
-
1
-
4
-
2
-
-
1
-
-
-
-
-
18
1
1
-
-
12
1
-
-
3
-
-
-
-
-
2
4
1
5
-
-
-
-
12
-
2
-
4
-
-
-
2
-
-
-
-
18
2
2
-
-
12
2
-
-
-
-
2
4
-
12
12