BRENDA - Enzyme Database
show all sequences of 2.7.1.173

Crystal structure of human nicotinamide riboside kinase

Khan, J.A.; Xiang, S.; Tong, L.; Structure 15, 1005-1013 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression of His-tagged NRK1 in Escherichia coli strain BL21(DE3)
Homo sapiens
Crystallization (Commentary)
Crystallization
Organism
NRK1 in complex with the reaction product nicotinamide mononucleotide, and NRK1 in a ternary complex with ADP and the anticancer drug tiazofurin, sitting-drop vapor-diffusion method, 22C, mixing of protein solution containing 20 mg/ml protein, 2 mM ligand, 20 mM Tris, pH 7.5-7.8, 200 mM NaCl, 5 mM DTT, with reservoir solution containing 28% w/v PEG 3350, 200 mM NH4Cl, 5 mM DTT, and 5 mM Na2HPO4, X-ray diffraction structure determination and analysis at 1.5 A and 2.7 A resolution, respectively, modeling
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + beta-D-ribosylnicotinamide
Homo sapiens
-
ADP + nicotinamide beta-D-ribonucleotide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9NWW6
-
-
Purification (Commentary)
Commentary
Organism
recombinant His-tagged NRK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Homo sapiens
Reaction
Reaction
Commentary
Organism
ATP + beta-D-ribosylnicotinate = ADP + nicotinate beta-D-ribonucleotide
catalytic mechanism, overview
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + beta-D-ribosylnicotinamide
-
682879
Homo sapiens
ADP + nicotinamide beta-D-ribonucleotide
-
-
-
?
ATP + nicotinamide mononucleotide
binding mode of the substrate, overview
682879
Homo sapiens
ADP + nicotinamide mononucleotide phosphate
-
-
-
?
ATP + tiazofurin
binding mode of the substrate, overview
682879
Homo sapiens
ADP + ?
-
-
-
?
additional information
ADP binding mode, overview
682879
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
More
the structure of NRK1 contains a central, five-stranded parallel beta sheet, with five alpha helices on both faces, overview
Homo sapiens
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
assay at
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of His-tagged NRK1 in Escherichia coli strain BL21(DE3)
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
NRK1 in complex with the reaction product nicotinamide mononucleotide, and NRK1 in a ternary complex with ADP and the anticancer drug tiazofurin, sitting-drop vapor-diffusion method, 22C, mixing of protein solution containing 20 mg/ml protein, 2 mM ligand, 20 mM Tris, pH 7.5-7.8, 200 mM NaCl, 5 mM DTT, with reservoir solution containing 28% w/v PEG 3350, 200 mM NH4Cl, 5 mM DTT, and 5 mM Na2HPO4, X-ray diffraction structure determination and analysis at 1.5 A and 2.7 A resolution, respectively, modeling
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + beta-D-ribosylnicotinamide
Homo sapiens
-
ADP + nicotinamide beta-D-ribonucleotide
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged NRK1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + beta-D-ribosylnicotinamide
-
682879
Homo sapiens
ADP + nicotinamide beta-D-ribonucleotide
-
-
-
?
ATP + nicotinamide mononucleotide
binding mode of the substrate, overview
682879
Homo sapiens
ADP + nicotinamide mononucleotide phosphate
-
-
-
?
ATP + tiazofurin
binding mode of the substrate, overview
682879
Homo sapiens
ADP + ?
-
-
-
?
additional information
ADP binding mode, overview
682879
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
More
the structure of NRK1 contains a central, five-stranded parallel beta sheet, with five alpha helices on both faces, overview
Homo sapiens
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.9
-
assay at
Homo sapiens
General Information
General Information
Commentary
Organism
physiological function
nicotinamide riboside kinase has an important role in the biosynthesis of NAD+ as well as the activation of tiazofurin and other nicotinamide riboside analogues for anticancer therapy
Homo sapiens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
nicotinamide riboside kinase has an important role in the biosynthesis of NAD+ as well as the activation of tiazofurin and other nicotinamide riboside analogues for anticancer therapy
Homo sapiens
Other publictions for EC 2.7.1.173
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739212
Sociali
Antitumor effect of combined N ...
Homo sapiens
Oncotarget
7
2968-2984
2016
-
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1
-
1
-
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1
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1
-
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2
-
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1
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1
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1
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1
-
-
-
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2
-
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1
-
-
-
-
-
-
-
-
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1
1
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-
738213
Zamporlini
Novel assay for simultaneous m ...
Homo sapiens
FEBS J.
281
5104-5119
2014
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1
-
-
-
-
-
-
-
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1
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8
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2
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1
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8
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2
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2
2
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698901
Belenky
Nicotinamide riboside and nico ...
Saccharomyces cerevisiae
J. Biol. Chem.
284
158-164
2009
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1
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2
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1
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2
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2
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2
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3
3
-
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-
718225
Matsui
Nicotinate riboside salvage in ...
Vigna radiata var. radiata
Plant Physiol. Biochem.
46
104-108
2008
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-
-
-
-
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1
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1
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1
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1
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3
1
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2
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1
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1
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1
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1
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1
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1
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1
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3
1
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2
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1
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1
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1
1
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682879
Khan
Crystal structure of human nic ...
Homo sapiens
Structure
15
1005-1013
2007
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1
1
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1
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1
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1
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1
1
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4
1
1
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1
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1
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1
1
1
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1
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1
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1
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4
1
1
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1
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1
1
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-
718250
Tempel
Nicotinamide riboside kinase s ...
Homo sapiens
PLoS Biol.
5
e263
2007
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1
1
5
-
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12
-
1
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4
-
2
-
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1
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-
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-
-
18
1
1
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12
1
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-
3
-
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-
2
4
1
5
-
-
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12
-
2
-
4
-
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2
-
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18
2
2
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12
2
-
-
-
-
2
4
-
12
12