BRENDA - Enzyme Database
show all sequences of 2.7.1.171

Human fructosamine-3-kinase: purification, sequencing, substrate specificity, and evidence of activity in vivo

Szwergold, B.S.; Howell, S.; Beisswenger, P.J.; Diabetes 50, 2139-2147 (2001)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
[protein]-N6-D-fructosyl-L-lysine
pH 8.0, 37°C
Homo sapiens
0.5
-
N5-D-fructosyl-L-ornithine
pH 8.0, 37°C
Homo sapiens
0.75
-
N6-D-fructosyl-L-lysine
pH 8.0, 37°C
Homo sapiens
50
-
D-fructose
pH 8.0, 37°C
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
x * 35000, calculated from sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + [protein]-N6-D-fructosyl-L-lysine
Homo sapiens
nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation
ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Reaction
Reaction
Commentary
Organism
ATP + [protein]-N6-D-fructosyl-L-lysine = ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine
phosphorylation destablilizes the fructoselysine adduct and leads to its spontaneous decomposition
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
erythrocyte
-
Homo sapiens
-
kidney
the enzyme is present in all tissues, with the highest level of expression in the kidney
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.08
-
pH 8.0, 37°C
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose
Vmax is 35% of the value for N6-D-fructosyl-L-lysine
708295
Homo sapiens
ADP + O3-phosphono-D-fructose
-
-
-
?
ATP + N5-D-fructosyl-L-ornithine
-
708295
Homo sapiens
ADP + N5-(O3-phosphono-D-fructosyl)-L-ornithine
-
-
-
?
ATP + N6-D-fructosyl-L-lysine
-
708295
Homo sapiens
ADP + N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
-
?
ATP + [protein]-N6-D-fructosyl-L-lysine
nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation
708295
Homo sapiens
ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
-
?
ATP + [protein]-N6-D-fructosyl-L-lysine
[histone]-N6-D-fructosyl-L-lysine, [hemoglobin]-N6-D-fructosyl-L-lysine. Similar experiments with other glycated proteins, including bovine serum albumin, and lysozyme indicate that fructoselysine residues on glycated proteins are readily phosphorylated by fructosamine 3-kinase, apparently irrespective of the protein. Phosphorylation destablilizes the fructoselysine adduct and leads to its spontaneous decomposition
708295
Homo sapiens
ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
-
?
additional information
the kinase is specific for 1-deoxy-1-amino fructose adducts and does not catalyze phosphorylation of other monosaccharides and polyols, such as glucose, galactose, mannose, glucosamine, galactosamine, or myo-inositol
708295
Homo sapiens
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 35000, calculated from sequence
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
[protein]-N6-D-fructosyl-L-lysine
pH 8.0, 37°C
Homo sapiens
0.5
-
N5-D-fructosyl-L-ornithine
pH 8.0, 37°C
Homo sapiens
0.75
-
N6-D-fructosyl-L-lysine
pH 8.0, 37°C
Homo sapiens
50
-
D-fructose
pH 8.0, 37°C
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
x * 35000, calculated from sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + [protein]-N6-D-fructosyl-L-lysine
Homo sapiens
nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation
ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
erythrocyte
-
Homo sapiens
-
kidney
the enzyme is present in all tissues, with the highest level of expression in the kidney
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.08
-
pH 8.0, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-fructose
Vmax is 35% of the value for N6-D-fructosyl-L-lysine
708295
Homo sapiens
ADP + O3-phosphono-D-fructose
-
-
-
?
ATP + N5-D-fructosyl-L-ornithine
-
708295
Homo sapiens
ADP + N5-(O3-phosphono-D-fructosyl)-L-ornithine
-
-
-
?
ATP + N6-D-fructosyl-L-lysine
-
708295
Homo sapiens
ADP + N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
-
?
ATP + [protein]-N6-D-fructosyl-L-lysine
nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation
708295
Homo sapiens
ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
-
?
ATP + [protein]-N6-D-fructosyl-L-lysine
[histone]-N6-D-fructosyl-L-lysine, [hemoglobin]-N6-D-fructosyl-L-lysine. Similar experiments with other glycated proteins, including bovine serum albumin, and lysozyme indicate that fructoselysine residues on glycated proteins are readily phosphorylated by fructosamine 3-kinase, apparently irrespective of the protein. Phosphorylation destablilizes the fructoselysine adduct and leads to its spontaneous decomposition
708295
Homo sapiens
ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
-
-
?
additional information
the kinase is specific for 1-deoxy-1-amino fructose adducts and does not catalyze phosphorylation of other monosaccharides and polyols, such as glucose, galactose, mannose, glucosamine, galactosamine, or myo-inositol
708295
Homo sapiens
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 35000, calculated from sequence
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Homo sapiens
Other publictions for EC 2.7.1.171
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738074
Cikomola
A simple colorimetric assay fo ...
Homo sapiens
Clin. Chem. Lab. Med.
55
154-159
2017
1
-
1
-
-
-
1
-
-
1
-
-
-
3
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
738073
Avemaria
Possible role of fructosamine ...
Homo sapiens
Clin. Chem. Lab. Med.
53
1315-1320
2015
-
-
1
-
1
-
1
-
-
1
-
1
-
2
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
738072
Hellwig
-
A new HPLC-based assay for the ...
Homo sapiens
Clin. Chem. Lab. Med.
52
93-101
2014
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
739258
Skrha
Fructosamine 3-kinase and glyo ...
Homo sapiens
Physiol. Res.
63 Suppl 2
S283-S291
2014
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
707094
van Schaftingen
Enzymatic repair of Amadori pr ...
Homo sapiens, Mus musculus
Amino Acids
42
1143-1150
2012
-
-
-
-
1
-
-
-
-
-
-
2
-
5
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
708589
Notarnicola
Reduced fructosamine-3-kinase ...
Homo sapiens
Genes Nutr.
5
257-262
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707061
Pascal
Effects of fructosamine-3-kina ...
Mus musculus
Am. J. Physiol. Endocrinol. Metab.
298
E586-596
2009
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
708421
Mohas
A polymorphism within the fruc ...
Homo sapiens
Exp. Clin. Endocrinol. Diabetes
118
209-212
2009
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710172
Caruso
Gene expression of fructosamin ...
Homo sapiens
Oncology
73
72-75
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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-
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
707098
Krause
A convenient HPLC assay for th ...
Homo sapiens
Anal. Bioanal. Chem.
386
2019-2025
2006
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
707426
Veiga da-Cunha
Increased protein glycation in ...
Mus musculus
Biochem. J.
399
257-264
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
708287
Delpierre
Variability in erythrocyte fru ...
Homo sapiens
Diabetes Metab.
32
31-39
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
707351
Conner
The expression of the genes fo ...
Homo sapiens
Biochem. Biophys. Res. Commun.
323
932-936
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
-
-
-
-
-
-
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-
-
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-
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-
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-
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-
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-
-
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-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
708296
Collard
A mammalian protein homologous ...
Homo sapiens, Mus musculus
Diabetes
52
2888-2895
2004
-
-
1
-
-
-
4
4
-
-
-
2
-
4
-
-
1
-
-
8
-
-
7
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
4
4
-
4
-
-
-
2
-
-
-
1
-
8
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708979
Delpierrre
Identification of fructosamine ...
Homo sapiens
J. Biol. Chem.
279
27613-27620
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
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-
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-
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1
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-
-
1
-
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2
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
708981
Delplanque
Tissue distribution and evolut ...
Gallus gallus, Mus musculus, Rattus norvegicus, Sus scrofa
J. Biol. Chem.
279
46606-46613
2004
-
-
-
-
-
-
-
-
-
-
-
3
-
12
-
-
3
-
-
16
-
-
6
-
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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