Literature summary for 2.7.1.17 extracted from

Bunker, R.D.; Bulloch, E.M.; Dickson, J.M.; Loomes, K.M.; Baker, E.N.
Structure and function of human xylulokinase, an enzyme with important roles in carbohydrate metabolism (2013), J. Biol. Chem., 288, 1643-1652.

Search for more literature for 2.7.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 200 mM MES-KOH, pH 6.0, 16% (w/v) polyethylene glycol 6000, 0.3 mM NaN3	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
5-deoxy-5-fluoro-D-xylulose	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.024	-	D-xylulose	in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O75191	-	-

Purification (Commentary)

Purification (Comment)	Organism
apo-enzyme and in complex with D-xylulose, ATP plus D-xylulose or 5-deoxy-5-fluoro-D-xylulose, nickel affinity column chromatography and Superdex 200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-xylulose	-	Homo sapiens	ADP + D-xylulose 5-phosphate	-	?
additional information	D-ribulose is not a substrate	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
D-xylulokinase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
35	-	D-xylulose	in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.025	-	5-deoxy-5-fluoro-D-xylulose	in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1500	-	D-xylulose	in 50 mM NH4HCO3, pH 8.0, 10% (v/v) D2O, 10 mM MgCl2 at 25°C	Homo sapiens

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Release 2023.1 (January 2023)