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Literature summary for 2.7.1.17 extracted from

  • Dmytruk, O.V.; Dmytruk, K.V.; Abbas, C.A.; Voronovsky, A.Y.; Sibirny, A.A.
    Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha (2008), Microb. Cell Fact., 7, 21.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant XYL3 gene driven by the HpGAP promotor transformed into the recipient strain H. polymorpha Ogataea angusta
recombinant XYL3 gene driven by the HpGAP promotor transformed into the recipient strain Hansenula polymorpha Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
strain CBS4732, thermotolerant, recombinant Hansenula polymorpha strain overexpressing the modified XR (K341R N343D) together with native XDH and XK on a DELTAxyl1 background
-
Ogataea angusta
-
overexpression of XK, thermotolerant
-
Ogataea angusta XRm/XDH/XK
-
overexpression of XK, thermotolerant
-
Ogataea angusta XRn/XDH/XK
-
overexpression of XK, thermotolerant
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
0.366 +/-0.019 U/mg protein, up to 2.4-fold increase in specific activity of XK as compared to wild type strain CBS4732, XK activity in cell extracts is determined spectrophotometrically at 37°C, XK assay mixture containes Tris-HCl buffer (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase (EC 1.1.1.27) 10 U, pyruvate kinase (EC 2.7.1.40) 0.05 U, and ATP 2 mM, reaction is started with addition of cell extract, blank without pyruvate kinase and lactate dehydrogenase is used to minimize interference of XDH activity in Hansenula polymorpha. Ogataea angusta
additional information
-
strain CBS4732, 0.156 +/-0.009 U/mg protein Ogataea angusta
additional information
-
strain XRn/XDH/XK, 0.375 +/-0.021 U/mg protein, up to 2.4-fold increase in specific activity of XK as compared to wild type strain CBS4732, activity in cell extracts is determined spectrophotometrically at 37°C, assay mixture containes Tris-HCl buffer (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase (EC 1.1.1.27) 10 U, pyruvate kinase (EC 2.7.1.40) 0.05 U, and ATP 2 mM. The reaction is started with addition of cell extract, blank without pyruvate kinase and lactate dehydrogenase is used to minimize interference of XDH activity in Hansenula polymorpha. Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose
-
Ogataea angusta ADP + D-xylulose 5-phosphate
-
r
ATP + D-xylulose
-
Ogataea angusta XRn/XDH/XK ADP + D-xylulose 5-phosphate
-
r
ATP + D-xylulose
-
Ogataea angusta XRm/XDH/XK ADP + D-xylulose 5-phosphate
-
r

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 48
-
Ogataea angusta