Cloned (Comment) | Organism |
---|---|
recombinant XYL3 gene driven by the HpGAP promotor transformed into the recipient strain H. polymorpha | Ogataea angusta |
recombinant XYL3 gene driven by the HpGAP promotor transformed into the recipient strain Hansenula polymorpha | Ogataea angusta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
strain CBS4732, thermotolerant, recombinant Hansenula polymorpha strain overexpressing the modified XR (K341R N343D) together with native XDH and XK on a DELTAxyl1 background | - |
Ogataea angusta | - |
overexpression of XK, thermotolerant | - |
Ogataea angusta XRm/XDH/XK | - |
overexpression of XK, thermotolerant | - |
Ogataea angusta XRn/XDH/XK | - |
overexpression of XK, thermotolerant | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.366 +/-0.019 U/mg protein, up to 2.4-fold increase in specific activity of XK as compared to wild type strain CBS4732, XK activity in cell extracts is determined spectrophotometrically at 37°C, XK assay mixture containes Tris-HCl buffer (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase (EC 1.1.1.27) 10 U, pyruvate kinase (EC 2.7.1.40) 0.05 U, and ATP 2 mM, reaction is started with addition of cell extract, blank without pyruvate kinase and lactate dehydrogenase is used to minimize interference of XDH activity in Hansenula polymorpha. | Ogataea angusta |
additional information | - |
strain CBS4732, 0.156 +/-0.009 U/mg protein | Ogataea angusta |
additional information | - |
strain XRn/XDH/XK, 0.375 +/-0.021 U/mg protein, up to 2.4-fold increase in specific activity of XK as compared to wild type strain CBS4732, activity in cell extracts is determined spectrophotometrically at 37°C, assay mixture containes Tris-HCl buffer (pH 7.8) 50 mM, MgCl2 5 mM, NADH 0.2 mM, phosphoenolpyruvate 1 mM, D-xylulose 8.5 mM, lactate dehydrogenase (EC 1.1.1.27) 10 U, pyruvate kinase (EC 2.7.1.40) 0.05 U, and ATP 2 mM. The reaction is started with addition of cell extract, blank without pyruvate kinase and lactate dehydrogenase is used to minimize interference of XDH activity in Hansenula polymorpha. | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | - |
Ogataea angusta | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Ogataea angusta XRn/XDH/XK | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + D-xylulose | - |
Ogataea angusta XRm/XDH/XK | ADP + D-xylulose 5-phosphate | - |
r |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 48 | - |
Ogataea angusta |