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Literature summary for 2.7.1.169 extracted from

  • Katoh, H.; Tamaki, H.; Tokutake, Y.; Hanada, S.; Chohnan, S.
    Identification of pantoate kinase and phosphopantothenate synthetase from Methanospirillum hungatei (2013), J. Biosci. Bioeng., 115, 372-376.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanospirillum hungatei

Inhibitors

Inhibitors Comment Organism Structure
malonyl-CoA 0.2 mM, 83% residual activity Methanospirillum hungatei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
Co2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
Fe2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
Mg2+ maximum activity in presence of Mg2+ Methanospirillum hungatei
Mn2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
additional information not accepted: Cu2+ Methanospirillum hungatei
Ni2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei
Zn2+ broadly accepts Ca2+, Fe2+, Co2+, Mn2+, Zn2+, or Ni2+ in place of Mg2+ Methanospirillum hungatei

Organism

Organism UniProt Comment Textmining
Methanospirillum hungatei Q2FUB2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantoate
-
Methanospirillum hungatei ADP + (R)-phosphopantoate
-
?
UTP + (R)-pantoate 52.7% of the activity with ATP Methanospirillum hungatei UDP + (R)-phosphopantoate
-
?

Synonyms

Synonyms Comment Organism
COG1829
-
Methanospirillum hungatei
Mhun_0831
-
Methanospirillum hungatei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Methanospirillum hungatei

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
no residual activity Methanospirillum hungatei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Methanospirillum hungatei

pH Range

pH Minimum pH Maximum Comment Organism
8 9.5 more than 80% of maximum activity Methanospirillum hungatei

General Information

General Information Comment Organism
physiological function coexpression of genes Mhun_0831 and Mhun_0832 complements the poor growth of the temperature-sensitive Escherichia coli pantothenate kinase mutant ts9. The recombinant Mhun_0831 and Mhun_0832 genes expressed in Escherichia coli cells exhibit pantoate kinase and phosphopantothenate synthetase activities, respectively Methanospirillum hungatei