Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Sulfurisphaera tokodaii |
expressed in Escherichia coli BL21(DE3) cells | Sulfurisphaera tokodaii |
General Stability | Organism |
---|---|
resistant to the 1 mM guanidine hydrochloride (85% of the original activity) and 1 mM NaF (65% of original activity) relatively stable in 10 mM MnCl2, CoSO4, CaCl2, SrCl2, NiCl2, CuCl2, HgCl2 | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-propanol | 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) | Sulfurisphaera tokodaii | |
Ca2+ | 85% activity in the presence of 10 mM Ca2+ compared to Mg2+ | Sulfurisphaera tokodaii | |
ethanol | 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) | Sulfurisphaera tokodaii | |
guanidine hydrochloride | 85% residual activity at 1 M guanidine hydrochloride | Sulfurisphaera tokodaii | |
Mn2+ | 93% activity in the presence of 10 mM Mn2+ compared to Mg2+ | Sulfurisphaera tokodaii | |
n-butanol | 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) | Sulfurisphaera tokodaii | |
Ni2+ | 68% activity in the presence of 10 mM Ni2+ compared to Mg2+ | Sulfurisphaera tokodaii | |
Phenylmethylsulfonylfluoride | less than 30% residual activity at 10 mM | Sulfurisphaera tokodaii | |
PMSF | 10 mM, more than 70% inhibition | Sulfurisphaera tokodaii | |
SDS | 10 mM, more than 60% inhibition; less than 40% residual activity at 10 mM | Sulfurisphaera tokodaii | |
Sodium fluoride | 65% residual activity at 1 M sodium fluoride | Sulfurisphaera tokodaii | |
Sr2+ | 79% activity in the presence of 10 mM Sr2+ compared to Mg2+ | Sulfurisphaera tokodaii | |
Zn2+ | 84% activity in the presence of 10 mM Zn2+ compared to Mg2+ | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent metal ion required, Ca2+ (10 mM) shows 85% of the activity compared to Mg2+ | Sulfurisphaera tokodaii | |
Co2+ | divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM) | Sulfurisphaera tokodaii | |
Co2+ | 106% activity in the presence of 10 mM Co2+ compared to Mg2+ | Sulfurisphaera tokodaii | |
Mg2+ | divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM) | Sulfurisphaera tokodaii | |
Mg2+ | 100% activity in the presence of 10 mM | Sulfurisphaera tokodaii | |
Mn2+ | divalent metal ion required, Mn2+ (10 mM) shows 93% of the activity compared to Mg2+ | Sulfurisphaera tokodaii | |
additional information | monovalent metal ions have no effect on the enzyme activity | Sulfurisphaera tokodaii | |
Ni2+ | divalent metal ion required, Ni2+ (10 mM) shows 68% of the activity compared to Mg2+ | Sulfurisphaera tokodaii | |
Sr2+ | divalent metal ion required, Sr2+ (10 mM) shows 79% of the activity compared to Mg2+ | Sulfurisphaera tokodaii | |
Zn2+ | divalent metal ion required, Zn2+ (10 mM) shows 84% of the activity compared to Mg2+ | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Sulfurisphaera tokodaii |
80000 | - |
gel filtration | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-glycerate | Sulfurisphaera tokodaii | key enzyme in the Entner-Doudoroff pathway in archaea | ADP + 2-phospho-(R)-glycerate | - |
? | |
ATP + (R)-glycerate | Sulfurisphaera tokodaii 7 | key enzyme in the Entner-Doudoroff pathway in archaea | ADP + 2-phospho-(R)-glycerate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q96YZ3 | - |
- |
Sulfurisphaera tokodaii 7 | Q96YZ3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Sulfurisphaera tokodaii |
recombinant enzyme | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + (R)-glycerate | 76% of the activity with GTP | Sulfurisphaera tokodaii | AMP + 2-phospho-(R)-glycerate | - |
? | |
ADP + (R)-glycerate | 76% of the activity with GTP | Sulfurisphaera tokodaii 7 | AMP + 2-phospho-(R)-glycerate | - |
? | |
ADP + D-glycerate | 76% activity compared to GTP | Sulfurisphaera tokodaii | AMP + 2-phospho-D-glycerate | - |
? | |
AMP + (R)-glycerate | 54% of the activity with GTP | Sulfurisphaera tokodaii | adenosine + 2-phospho-(R)-glycerate | - |
? | |
AMP + D-glycerate | 54% activity compared to GTP | Sulfurisphaera tokodaii | adenosine + 2-phospho-D-glycerate | - |
? | |
ATP + (R)-glycerate | key enzyme in the Entner-Doudoroff pathway in archaea | Sulfurisphaera tokodaii | ADP + 2-phospho-(R)-glycerate | - |
? | |
ATP + (R)-glycerate | 92% of the activity with GTP | Sulfurisphaera tokodaii | ADP + 2-phospho-(R)-glycerate | - |
? | |
ATP + (R)-glycerate | key enzyme in the Entner-Doudoroff pathway in archaea | Sulfurisphaera tokodaii 7 | ADP + 2-phospho-(R)-glycerate | - |
? | |
ATP + (R)-glycerate | 92% of the activity with GTP | Sulfurisphaera tokodaii 7 | ADP + 2-phospho-(R)-glycerate | - |
? | |
ATP + D-glycerate | 92% activity compared to GTP | Sulfurisphaera tokodaii | ADP + 2-phospho-D-glycerate | - |
? | |
CTP + (R)-glycerate | 87% of the activity with GTP | Sulfurisphaera tokodaii | CDP + 2-phospho-(R)-glycerate | - |
? | |
CTP + (R)-glycerate | 87% of the activity with GTP | Sulfurisphaera tokodaii 7 | CDP + 2-phospho-(R)-glycerate | - |
? | |
CTP + D-glycerate | 87% activity compared to GTP | Sulfurisphaera tokodaii | CDP + 2-phospho-D-glycerate | - |
? | |
diphosphate + (R)-glycerate | 63% of the activity with ATP | Sulfurisphaera tokodaii | phosphate + 2-phospho-(R)-glycerate | - |
? | |
diphosphate + (R)-glycerate | 63% of the activity with ATP | Sulfurisphaera tokodaii 7 | phosphate + 2-phospho-(R)-glycerate | - |
? | |
diphosphate + D-glycerate | 63% activity compared to GTP | Sulfurisphaera tokodaii | phosphate + 2-phospho-D-glycerate | - |
? | |
GTP + (R)-glycerate | - |
Sulfurisphaera tokodaii | GDP + 2-phospho-(R)-glycerate | - |
? | |
GTP + D-glycerate | 100% activity towards GTP | Sulfurisphaera tokodaii | GDP + 2-phospho-D-glycerate | - |
? | |
UTP + (R)-glycerate | 81% of the activity with GTP | Sulfurisphaera tokodaii | UDP + 2-phospho-(R)-glycerate | - |
? | |
UTP + D-glycerate | 81% activity compared to GTP | Sulfurisphaera tokodaii | UDP + 2-phospho-D-glycerate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 45000, SDS-PAGE | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | - |
Sulfurisphaera tokodaii |
70 | 100 | 70°C: about 60% of maximal activity, 100°C: about 85% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
12 h, more than 85% of the activity remains | Sulfurisphaera tokodaii |
100 | - |
the enzyme activity shows no obvious decrease even after heat-treatment at 100°C for 12 h and most of the optimum activity (more than 85%) remains | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | pH 3: about 85% of maximal activity, pH 9.0: about 50% of maximal activity | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Sulfurisphaera tokodaii |