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Literature summary for 2.7.1.165 extracted from

  • Kehrer, D.; Ahmed, H.; Brinkmann, H.; Siebers, B.
    Glycerate kinase of the hyperthermophilic archaeon Thermoproteus tenax: new insights into the phylogenetic distribution and physiological role of members of the three different glycerate kinase classes (2007), BMC Genomics, 8, 301.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.165

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermoproteus tenax

Inhibitors

Inhibitors Comment Organism Structure
(R)-glycerate substrate inhibition at high glycerate concentrations Thermoproteus tenax
ADP competitive inhibition Thermoproteus tenax
EDTA 40 mM Thermoproteus tenax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 (R)-glycerate
-
 Thermoproteus tenax
0.03
-
 ATP
-
 Thermoproteus tenax

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 15% of the activity with Mg2+ Thermoproteus tenax
Co2+ 56% of the activity with Mg2+ Thermoproteus tenax
Mg2+ highest activity is observed in presence of 20 mM Mg2+ Thermoproteus tenax
Mn2+ 59% of the activity with Mg2+ Thermoproteus tenax
additional information no activity is detected in the presence of Cu2+ and Fe2+ at 0.2, 2, 5 and 20 mM, respectively Thermoproteus tenax
Ni2+ 30% of the activity with Mg2+ Thermoproteus tenax

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 1 * 44000, SDS-PAGE Thermoproteus tenax
48300
-
 gel filtration Thermoproteus tenax

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-glycerate Thermoproteus tenax enzyme of the branched Entner-Doudoroff pathway ADP + 2-phospho-(R)-glycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoproteus tenax Q703V9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoproteus tenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-glycerate
-
 Thermoproteus tenax ADP + 2-phospho-(R)-glycerate
-
 ?
ATP + (R)-glycerate enzyme of the branched Entner-Doudoroff pathway Thermoproteus tenax ADP + 2-phospho-(R)-glycerate
-
 ?
CTP + (R)-glycerate 16% of the activity with ATP Thermoproteus tenax CDP + 2-phospho-(R)-glycerate
-
 ?
GTP + (R)-glycerate 20% of the activity with ATP Thermoproteus tenax GDP + 2-phospho-(R)-glycerate
-
 ?
additional information no activity is observed with ADP, diphosphate and polyphosphates Thermoproteus tenax ?
-
 ?
TTP + (R)-glycerate 16% of the activity with ATP Thermoproteus tenax TDP + 2-phospho-(R)-glycerate
-
 ?
UTP + (R)-glycerate 10% of the activity with ATP Thermoproteus tenax UDP + 2-phospho-(R)-glycerate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44000, SDS-PAGE Thermoproteus tenax

Synonyms

Synonyms Comment Organism
garK
-
 Thermoproteus tenax

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermoproteus tenax

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 90 50°C: 15% of maximal activity, 70°C: 55% of maximal activity, 90°C: maximal activity Thermoproteus tenax

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1
-
 ATP
-
 Thermoproteus tenax
3.6
-
 D-glycerate
-
 Thermoproteus tenax