KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
(R)-glycerate | - |
Picrophilus torridus | |
0.5 | - |
ATP | - |
Picrophilus torridus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 0.05 mM, most effective divalent cation | Picrophilus torridus | |
additional information | Mg2+ can be replaced to some extent by Ni2+ (25%), Mn2+ (11%), and Co2+ (11%) | Picrophilus torridus | |
additional information | no activating effect of K+ | Picrophilus torridus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47500 | - |
2 * 47500, gel filtration | Picrophilus torridus |
50000 | - |
SDS-PAGE, denatured enzyme | Picrophilus torridus |
93000 | - |
calculated from amino acid sequence | Picrophilus torridus |
95000 | - |
gel filtration, native enzyme | Picrophilus torridus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Picrophilus torridus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Resource Phenyl column chromatography, Superdex 200 HiLoad 16/60 column gel filtration, and UNO-Q1 column chromatography | Picrophilus torridus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
cell extract, at 50°C | Picrophilus torridus |
358 | - |
after 5114fold purification, at 50°C | Picrophilus torridus |
Storage Stability | Organism |
---|---|
60°C, 100 mM Tris-HCl at pH 8.0, 2 h, no loss of activity | Picrophilus torridus |
70°C, 100 mM Tris-HCl at pH 8.0, 5 min, 50% loss of activity | Picrophilus torridus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-glycerate | - |
Picrophilus torridus | ADP + 3-phospho-(R)-glycerate | - |
? | |
additional information | the enzyme shows highest activity with D-glycerate (100%), and lower activity (34%) with L-glycerate | Picrophilus torridus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 47500, gel filtration | Picrophilus torridus |
Synonyms | Comment | Organism |
---|---|---|
GCK | - |
Picrophilus torridus |
glycerate kinase (2-phosphoglycerate forming) | - |
Picrophilus torridus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Picrophilus torridus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Picrophilus torridus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.6 | 8.6 | 50% of activity at pH 5.6 and at pH 8.6 | Picrophilus torridus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Picrophilus torridus |