Literature summary for 2.7.1.165 extracted from

Yoshida, T.; Fukuta, K.; Mitsunaga, T.; Yamada, H.; Izumi, Y.
Purification and characterization of glycerate kinase from a serine-producing methylotroph, Hyphomicrobium methylovorum GM2 (1992), Eur. J. Biochem., 210, 849-854.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2,3-diphospho-D-glycerate	-	Hyphomicrobium methylovorum
3-phosphoglycerate	-	Hyphomicrobium methylovorum
AgNO3	-	Hyphomicrobium methylovorum
CuCl2	-	Hyphomicrobium methylovorum
HgCl2	-	Hyphomicrobium methylovorum
Hydroxypyruvate	-	Hyphomicrobium methylovorum
L-Glycerate	-	Hyphomicrobium methylovorum
p-chloromercuribenzoate	-	Hyphomicrobium methylovorum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.13	-	ATP	cosubstrate D-glycerate, at pH 7.0, 30°C	Hyphomicrobium methylovorum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	10 mM, 75% of the activity with Mg2+	Hyphomicrobium methylovorum
K+	50 mM, high stimulation	Hyphomicrobium methylovorum
Mg2+	required	Hyphomicrobium methylovorum
Mg2+	10 mM, activates	Hyphomicrobium methylovorum
Mn2+	10 mM, 72% of the activity with 10 mM Mg2+	Hyphomicrobium methylovorum
NH4+	50 mM, high stimulation	Hyphomicrobium methylovorum
Ni2+	stimulates, 10 mM, 29% of the activity with Mg2+	Hyphomicrobium methylovorum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41000	48000	gel filtration	Hyphomicrobium methylovorum
52000	-	1 * 52000, SDS-PAGE	Hyphomicrobium methylovorum

Organism

Organism	UniProt	Comment	Textmining
Hyphomicrobium methylovorum	-	GM2	-

Purification (Commentary)

Purification (Comment)	Organism
-	Hyphomicrobium methylovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
181	-	pH 7.0, 30°C	Hyphomicrobium methylovorum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (R)-glycerate	activity towards L-glycerate is 13% of that towards D-glycerate	Hyphomicrobium methylovorum	ADP + 3-phospho-(R)-glycerate	2-phosphoglycerate	ir
CTP + (R)-glycerate	at 59% of the activity with ATP	Hyphomicrobium methylovorum	CDP + 3-phospho-(R)-glycerate	-	?
GTP + (R)-glycerate	at 59% of the activity with ATP	Hyphomicrobium methylovorum	GDP + 3-phospho-(R)-glycerate	-	?
UTP + (R)-glycerate	at 64% of the activity with ATP	Hyphomicrobium methylovorum	UDP + 3-phospho-(R)-glycerate	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 52000, SDS-PAGE	Hyphomicrobium methylovorum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Hyphomicrobium methylovorum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
35	-	30 min, stable	Hyphomicrobium methylovorum
40	-	30 min, 14.3% loss of activity	Hyphomicrobium methylovorum
50	-	30 min, 52.4% loss of activity	Hyphomicrobium methylovorum
60	-	30 min, 87.3% loss of activity	Hyphomicrobium methylovorum
70	-	30 min, 100% loss of activity	Hyphomicrobium methylovorum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	-	Hyphomicrobium methylovorum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	10	30°C, 30 min, stable	Hyphomicrobium methylovorum

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Release 2023.1 (January 2023)