Application | Comment | Organism |
---|---|---|
molecular biology | Used as marker gene mediating hygromycin resistance. | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
hygromycin B resistance gene is cloned in pBR322, recombinant plasmids pKC241 pKC222 carrying the resistance | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | included in assay medium | Escherichia coli | |
NH4+ | included in assay medium | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + hygromycin B | Escherichia coli | phosphorylated product procures hygromcin B resistance | ADP + 4-O-phosphohygromycin B | - |
ir | |
additional information | Escherichia coli | no phosphorylation of neomycin, kanamycin A and B, streptomycin, dideoxykanamycin B, tobramycin, gentamicin, G418, sisomicin, netilmicin, amikacin, apramycin, ribostamycin, butirosin, lividomycin, and paromomycin by this enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
used strains are K-12 derivatives, isolating of a plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strains W677, BE783, BE827, DH1, BE1065, BE1092, and BE1098 are used | Escherichia coli | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
107471 counts/minute/25 microl sample extract, hygromycin as substrate | Escherichia coli |
additional information | - |
14328 counts/minute/25 microl sample extract, A23444 as substrate | Escherichia coli |
additional information | - |
15508 counts/minute/25 microl sample extract, SS-56C as substrate | Escherichia coli |
additional information | - |
24483 counts/minute/25 microl sample extract, destomycin A as substrate | Escherichia coli |
additional information | - |
35387 counts/minute/25 microl sample extract, strain BE1065 containing plasmid pKC222 | Escherichia coli |
additional information | - |
50051 counts/minute/25 microl sample extract, strain BE1098 containing plasmid pKC241 | Escherichia coli |
additional information | - |
5986 counts/minute/25 microl sample extract, hygromycin b2 as substrate | Escherichia coli |
additional information | - |
8428 counts/minute/25 microl sample extract, destomycin B as substrate | Escherichia coli |
additional information | - |
hygromycin B is an aminocyclitol antibiotic with broad-spectrum activity against both procaryotic and eucaryotic cells | Escherichia coli |
additional information | - |
No activity is found in the plasmid-free DH1 strain. | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + destomycin A | 1-N-methyl isomer of hygromycin B | Escherichia coli | ADP + 4-O-phosphodestomycin A | - |
? | |
ATP + destomycin B | 1-N-methyl-4',4"-epi hygromycin B | Escherichia coli | ADP + 4-O-phosphodestomycin B | - |
? | |
ATP + hygromycin B | phosphorylated product procures hygromcin B resistance | Escherichia coli | ADP + 4-O-phosphohygromycin B | - |
ir | |
ATP + hygromycin B2 | pseudodisaccharide of D-talose and hyosamine | Escherichia coli | ADP + 4-O-phosphohygromycin B2 | - |
? | |
additional information | no phosphorylation of neomycin, kanamycin A and B, streptomycin, dideoxykanamycin B, tobramycin, gentamicin, G418, sisomicin, netilmicin, amikacin, apramycin, ribostamycin, butirosin, lividomycin, and paromomycin by this enzyme | Escherichia coli | ? | - |
? | |
additional information | the analogs of destomycin A and B, A23444 (N-demethyl hygromycin B) and SS-56C (N-demethyl-2-hydroxy hygromycin B) with modifications in the cyclitol ring moiety, are phosphoryted by the enzyme | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hm phosphotransferase | - |
Escherichia coli |
hygromycin B4-O-phosphohygromycin | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |