Cloned (Comment) | Organism |
---|---|
Fusion of the coding region of the splicing enzyme in-frame to the amino-terminus of the green fluorescent protein (GFP). Full-length 2'-phosphotransferase cDNA (289 aa) and a short version of the same polypeptide starting with the second methionine at position 33 are fused to the coding region of GFP. Additionally, the N-terminal sequence of 97 amino acids derived from the 2'-phosphotransferase is directly fused to GFP. | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | enzyme contains chloroplast transit signals at the N-terminus, 2'-phosphotransferase are not required for classical tRNA splicing in chloroplast, it has an alternative function | Arabidopsis thaliana | 9507 | - |
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
mitochondrion | 2'-phosphotransferase shows a tendency to associate with mitochondria | Arabidopsis thaliana | 5739 | - |
nucleus | - |
Arabidopsis thaliana | 5634 | - |
proplastid | - |
Arabidopsis thaliana | 9537 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-phospho-(ligated tRNA) + NAD+ | Arabidopsis thaliana | last step in the pre-tRNA splicing process | mature tRNA + ADP-ribose1'',2''-phosphate + nicotinamide + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
plant splicing enzymes fused to GFP by their transient expression in Allium epidermal and Vicia guard cells. | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | Upper epidermis of Allium cepa is used for transient expression | Arabidopsis thaliana | - |
leaf epidermis | Lower leaf epidermis from Vicia faba is used for transient expression | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-phospho-(ligated tRNA) + NAD+ | last step in the pre-tRNA splicing process | Arabidopsis thaliana | mature tRNA + ADP-ribose1'',2''-phosphate + nicotinamide + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | in plants | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
2'-phosphotransferase | removes 2'-phosphate from the splice junction of the precursor t-RNA | Arabidopsis thaliana |
AtPt | - |
Arabidopsis thaliana |
NAD+-dependent 2'-phosphotransferase | - |
Arabidopsis thaliana |
Tpt1 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana |