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Literature summary for 2.7.1.160 extracted from

  • Sawaya, R.; Schwer, B.; Shuman, S.
    Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1 (2005), RNA, 11, 107-113.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.160

Cloned(Commentary)

Cloned (Comment) Organism
expression from Saccharomyces cerevisiae vectors Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae Escherichia coli

Protein Variants

Protein Variants Comment Organism
F72A temperature sensitive Saccharomyces cerevisiae
H117A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
H117A-T119A lethal in vivo Saccharomyces cerevisiae
H142A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
H22A lethal in vivo Escherichia coli
H24A lethal in vivo Saccharomyces cerevisiae
H24N lethal in vivo Saccharomyces cerevisiae
H24Q lethal in vivo Saccharomyces cerevisiae
H90A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
K16A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
K69A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
R125A lethal in vivo Escherichia coli
R138A lethal in vivo Saccharomyces cerevisiae
R138K colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
R138Q lethal in vivo Saccharomyces cerevisiae
R158A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
R21A lethal in vivo Escherichia coli
R23A lethal in vivo Saccharomyces cerevisiae
R23K lethal in vivo Saccharomyces cerevisiae
R23Q lethal in vivo Saccharomyces cerevisiae
R69A lethal in vivo Escherichia coli
R71A lethal in vivo Saccharomyces cerevisiae
R71K lethal in vivo Saccharomyces cerevisiae
R71Q grows slower than wild-type, temperature sensitive Saccharomyces cerevisiae
S91A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
T119A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae
Y38A colony size indistinguishable from that of wild-type TPT1 strain Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix
-
-
-
Archaeoglobus fulgidus
-
-
-
Clostridium perfringens
-
-
-
Drosophila melanogaster
-
-
-
Escherichia coli
-
-
-
Homo sapiens
-
-
-
Leishmania major
-
-
-
Nostoc punctiforme
-
-
-
Pyrococcus horikoshii
-
-
-
Saccharomyces cerevisiae
-
-
-
Schizosaccharomyces pombe
-
-
-
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-agarose chromatography Escherichia coli
Ni-agarose chromatography Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-80, Tris-HCl buffer, pH 8.0, 10% glycerol Saccharomyces cerevisiae

Subunits

Subunits Comment Organism
monomer SDS-PAGE Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
KptA
-
 Escherichia coli
Tpt1
-
 Saccharomyces cerevisiae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
18 37 growth of mutants K16A, K69A, H90A, S91A, H117A, T119A, H142A, R158A and R138K on yeast medium/peptone/dextrose agar, colony sizes were similar to that of wild-type TPT1 cells Saccharomyces cerevisiae
18 30 F27A, R71Q, TPT1(1-220) and TPT1(1-210) cells failed to grow at 37°C on yeast medium/peptone/dextrose agar, R71Q and TPT1(1-210) strains grew slower than wild-type TPT1 Saccharomyces cerevisiae
25 30 growth of mutants K16A, Y38A, K69A, F72A, H90A, S91A, H117A, T119A, H142A, R158A, TPT1(1-220) and TPT1(1-210) on 5-fluoroorotic acid agar medium Saccharomyces cerevisiae