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Literature summary for 2.7.1.160 extracted from

  • Spinelli, S.L.; Kierzek, R.; Turner, D.H.; Phizicky, E.M.
    Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast (1999), J. Biol. Chem., 274, 2637-2644.
    View publication on PubMed
Search for more literature for 2.7.1.160

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus
expression in Escherichia coli Escherichia coli
expression in Escherichia coli Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mus musculus
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by Blue Sepharose column chromatography Mus musculus
by Blue Sepharose column chromatography Escherichia coli
by nickel affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-phospho-[ligated tRNA] + NAD+ mTpt1 can catalyze the conversion of the KptA-generated intermediate to both product and the original substrate Mus musculus mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?
2'-phospho-[ligated tRNA] + NAD+ production of a reaction intermediate, nicotinamide release occurs during intermediate formation, KptA can catalyze the conversion of the intermediate to both product and the original substrate Escherichia coli mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?
2'-phospho-[ligated tRNA] + NAD+ Tpt1 can catalyze the conversion of the KptA-generated intermediate to both product and the original substrate Saccharomyces cerevisiae mature tRNA + ADP ribose-1'',2''-phosphate + nicotinamide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
KptA
-
 Escherichia coli
mTPT1
-
 Mus musculus
Tpt1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Saccharomyces cerevisiae
37
-
-
 Mus musculus
37
-
-
 Escherichia coli