Literature summary for 2.7.1.159 extracted from

Marathe, A.; Krishnan, V.; Vinutha, T.; Dahuja, A.; Jolly, M.; Sachdev, A.
Exploring the role of inositol 1,3,4-trisphosphate 5/6 kinase-2 (GmITPK2) as a dehydration and salinity stress regulator in Glycine max (L.) Merr. through heterologous expression in E. coli (2018), Plant Physiol. Biochem., 123, 331-341 .

Search for more literature for 2.7.1.159

Cloned(Commentary)

Cloned (Comment)	Organism
gene Itpk2, genetic organization and gene structure, isozyme sequence comparisons, in-silico analysis of the promoter region of ITPKs, the promoter region of GmITPK2 shows the presence of two MYB binding elements for drought inducibility and one for abscisic acid response, quantitative RT-PCR enzyme expression analysis. The His5-tagged GmITPK2-expressing recombinant Escherichia coli strain BL21(DE3) cells show tolerance to drought and salinity and thus demonstrate the functional redundancy of the gene across taxa	Glycine max

Cloned (Comment)

Organism

gene Itpk2, genetic organization and gene structure, isozyme sequence comparisons, in-silico analysis of the promoter region of ITPKs, the promoter region of GmITPK2 shows the presence of two MYB binding elements for drought inducibility and one for abscisic acid response, quantitative RT-PCR enzyme expression analysis. The His5-tagged GmITPK2-expressing recombinant Escherichia coli strain BL21(DE3) cells show tolerance to drought and salinity and thus demonstrate the functional redundancy of the gene across taxa

Glycine max

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Glycine max	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 1D-myo-inositol 1,3,4-trisphosphate	Glycine max	-	ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate	-	?
ATP + 1D-myo-inositol 1,3,4-trisphosphate	Glycine max	-	ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Glycine max	A7X665	cv. Pusa-16, grown in the fields of Division of Genetics, I.A.R.I, New Delhi	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His5-tagged GmITPK2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Glycine max

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Glycine max	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 1D-myo-inositol 1,3,4-trisphosphate	-	Glycine max	ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate	-	?
ATP + 1D-myo-inositol 1,3,4-trisphosphate	-	Glycine max	ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 35000, recombinant His5-tagged enzyme, SDS-PAGE	Glycine max

Synonyms

Synonyms	Comment	Organism
GmItpk2	-	Glycine max
inositol 1,3,4-trisphosphate 5/6 kinase-2	-	Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	in vivo assay at	Glycine max

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Glycine max

Expression

Organism	Comment	Expression
Glycine max	expression profiling of the GmITPK transcripts under different experimental conditions of drought and salinity stress. Plants treated with 200 mM NaCl, the transcript expression of GmITPK2 is maximal 1 h after treatment i.e. 7.09fold and gradually reduced to 2.82fold 8 h after treatment. A steady upregulation is observed in plants treated with 0.1 mM abscisic acid as the transcript accumulation increased gradually with time ranging from 1.0 to 14fold. In PEG-induced drought stress the transcript accumulation is maximal (6.6fold) 1 h after treatment and steadily decreases to 3.4 8 h after treatment. The transcript profiles show a higher fold change in the expression GmITPK2 alone, compared to remaining members of GmITPK gene family	additional information

General Information

General Information	Comment	Organism
physiological function	inositol 1,3,4, trisphosphate 5/6 kinase (ITPK), a polyphosphate kinase that converts inositol 1,3,4-trisphosphate to inositol 1,3,4,5/6-tetraphosphate, averting the inositol phosphate pool towards phytic acid (PA) biosynthesis, is a key regulator that exists in four different isoforms in soybean. Role of inositol 1,3,4-trisphosphate 5/6 kinase-2 (GmITPK2) as a dehydration and salinity stress regulator in Glycine max. The significantly higher expression of GmITPK2 under drought and salinity stress suggests its role in providing tolerance mechanism against abiotic stress	Glycine max