Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-AI cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D368A | inactive | Arabidopsis thaliana |
H196A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
K168A | inactive | Arabidopsis thaliana |
K170A | inactive | Arabidopsis thaliana |
K200A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
K411A | inactive | Arabidopsis thaliana |
N238A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
N239A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
R130A | inactive | Arabidopsis thaliana |
R192A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
R415A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
R45A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
Y419A | the mutant shows reduced activity compared to the wild type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03372 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme N238A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.03979 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme K200A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.04348 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme H196A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.04754 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.0548 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme R45A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.05599 | - |
1D-myo-inositol 1,4,5,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.05935 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme R192A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.06227 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme R415A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.06305 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.06681 | - |
1D-myo-inositol 1,3,4,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.07686 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme Y419A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.0833 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme N239A, at pH 7.5 and 25°C | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | Arabidopsis thaliana | - |
ADP + 1D-myo-inositol hexakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93YN9 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA bead chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate | - |
Arabidopsis thaliana | ? | - |
? | |
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
Arabidopsis thaliana | ? | - |
? | |
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate | - |
Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with 1D-myo-inositol 1,3,4,5-tetrakisphosphate | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol 1,3,4,5,6-pentakisphosphate 2-kinase | - |
Arabidopsis thaliana |
Ipk1 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.105 | - |
1D-myo-inositol 3,4,5,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.11 | - |
1D-myo-inositol 1,4,5,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.246 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme N238A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.265 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme N239A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.279 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme K200A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.367 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme R192A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.453 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme R45A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.4581 | - |
1D-myo-inositol 1,3,4,6-tetrakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.569 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme H196A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.645 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme R415A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.694 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | mutant enzyme Y419A, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.734 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |