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Literature summary for 2.7.1.158 extracted from

  • Gosein, V.; Miller, G.J.
    Roles of phosphate recognition in inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPK1) substrate binding and activation (2013), J. Biol. Chem., 288, 26908-26913.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-AI cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D368A inactive Arabidopsis thaliana
H196A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
K168A inactive Arabidopsis thaliana
K170A inactive Arabidopsis thaliana
K200A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
K411A inactive Arabidopsis thaliana
N238A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
N239A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
R130A inactive Arabidopsis thaliana
R192A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
R415A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
R45A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana
Y419A the mutant shows reduced activity compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03372
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme N238A, at pH 7.5 and 25°C Arabidopsis thaliana
0.03979
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme K200A, at pH 7.5 and 25°C Arabidopsis thaliana
0.04348
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme H196A, at pH 7.5 and 25°C Arabidopsis thaliana
0.04754
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.0548
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme R45A, at pH 7.5 and 25°C Arabidopsis thaliana
0.05599
-
1D-myo-inositol 1,4,5,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.05935
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme R192A, at pH 7.5 and 25°C Arabidopsis thaliana
0.06227
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme R415A, at pH 7.5 and 25°C Arabidopsis thaliana
0.06305
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.06681
-
1D-myo-inositol 1,3,4,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.07686
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme Y419A, at pH 7.5 and 25°C Arabidopsis thaliana
0.0833
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme N239A, at pH 7.5 and 25°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Arabidopsis thaliana
-
ADP + 1D-myo-inositol hexakisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93YN9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA bead chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol hexakisphosphate
-
?
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
Arabidopsis thaliana ?
-
?
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
Arabidopsis thaliana ?
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
-
Arabidopsis thaliana ?
-
?
additional information no activity with 1D-myo-inositol 1,3,4,5-tetrakisphosphate Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
inositol 1,3,4,5,6-pentakisphosphate 2-kinase
-
Arabidopsis thaliana
Ipk1
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.105
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.11
-
1D-myo-inositol 1,4,5,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.246
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme N238A, at pH 7.5 and 25°C Arabidopsis thaliana
0.265
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme N239A, at pH 7.5 and 25°C Arabidopsis thaliana
0.279
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme K200A, at pH 7.5 and 25°C Arabidopsis thaliana
0.367
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme R192A, at pH 7.5 and 25°C Arabidopsis thaliana
0.453
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme R45A, at pH 7.5 and 25°C Arabidopsis thaliana
0.4581
-
1D-myo-inositol 1,3,4,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.569
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme H196A, at pH 7.5 and 25°C Arabidopsis thaliana
0.645
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme R415A, at pH 7.5 and 25°C Arabidopsis thaliana
0.694
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme Y419A, at pH 7.5 and 25°C Arabidopsis thaliana
0.734
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana