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Literature summary for 2.7.1.158 extracted from
- A Solanum tuberosum inositol phosphate kinase (StITPK1) displaying inositol phosphate-inositol phosphate and inositol phosphate-ADP phosphotransferase activities (2008), FEBS Lett., 582, 1731-1737.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Cloning from a potato tuber cDNA library and expression of StITPK1-His protein in Escherichia coli rosetta cells. | Solanum tuberosum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | included in assay medium | Solanum tuberosum |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | Solanum tuberosum | the predominant activity of StITPK1 is that of an InsP5-ADP phosphotransferase. | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
| additional information | Solanum tuberosum | StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate-ADP phosphotransferase activity in presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | A4H2J0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by batch-elution from Ni-NTA affinity resin, Analysis by SDS-PAGE gel and Western Blot using an anti-His antibody. Purity of protein is >98% estimated by SDS-PAGE. | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | expression of Solanum tuberosum protein in Escherichia coli | Solanum tuberosum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | the predominant activity of StITPK1 is that of an InsP5-ADP phosphotransferase. | Solanum tuberosum | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
| additional information | StITPK1 displays inositol 3,4,5,6-tetrakisphosphate 1-kinase activity. The enzyme displays inositol 1,3,4,5,6-pentakisphosphate 1-phosphatase activity in the absence of a nucleotide acceptor and inositol 1,3,4,5,6-pentakisphosphate-ADP phosphotransferase activity in presence of physiological concentrations of ADP. Additionally, StITPK1 shows inositol phosphate-inositol phosphate phosphotransferase activity. | Solanum tuberosum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inositol polyphosphate kinase-1 | - |
Solanum tuberosum |
| inositol polyphosphate kinase/phosphotransferase | multifunctional | Solanum tuberosum |
| StITPK1 | - |
Solanum tuberosum |
| StITPKalpha | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Solanum tuberosum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Solanum tuberosum | |
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Release 2023.1 (January 2023)
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