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Literature summary for 2.7.1.158 extracted from
- Purification and some properties of inositol 1,3,4,5,6-pentakis phosphate 2-kinase from immature soybean seeds (1994), J. Biol. Chem., 269, 28393-28399.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | 0.0025%, increases activity of purified enzyme by 40% | Glycine max | |
| Triton X-100 | 0.01%, increases activity | Glycine max |  |
| Tween 20 | 0.01%, increases activity | Glycine max | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | slightly inhibitory | Glycine max | |
| Ca2+ | 20 mM, complete inhibition | Glycine max | |
| KCl | strongly inhibitory at 200 mM | Glycine max | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0023 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Glycine max | |
| 0.0084 | - |
ATP | - |
Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity at 4 mM | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, SDS-PAGE | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | Glycine max | the enzyme may be involved in both inositol hexakisphosphate formation in maturing seeds and ATP resynthesis in germinating seeds | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | immature | Glycine max | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, enzyme retains 50% of its activity after 6 weeks | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Glycine max | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | the enzyme may be involved in both inositol hexakisphosphate formation in maturing seeds and ATP resynthesis in germinating seeds | Glycine max | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
| ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
Glycine max | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, SDS-PAGE | Glycine max |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inositol 1,3,4,5,6-pentakis phosphate 2-kinase | - |
Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Glycine max |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 60% of maximal activity, 50°C: about 10% of maximal activity | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Glycine max |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | about 50% of maximal activity at pH 5.5 and pH 8.0 | Glycine max |
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