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Literature summary for 2.7.1.154 extracted from
- The class II phosphoinositide 3-kinases PI3K-C2alpha and PI3K-C2beta differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells (2019), J. Physiol. Sci., 69, 263-280 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ITSN1 | endogenous ITSN1 is required for the formation of actin patches and recruitment of PI3K-C2beta to the clathrin-coated structures | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | siRNA-mediated knockdown of PI3K protein expression in human umbilical vein endothelial cells | Homo sapiens |
| additional information | siRNA-mediated knockdown of PI3K protein expression in human umbilical vein endothelial cells. mCherry-C2beta overexpression stimulates pinocytosis through the mechanisms involving GFP-ITSN1-mediated recruitment of mCherry-C2beta | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| actin filament | - |
Homo sapiens | 5884 | - |
| clathrin-coated vesicle | - |
Homo sapiens | 30136 | - |
| clathrin-coated vesicle | PI3K-C2alpha is closely associated with clathrin-coated pits and vesicles through its direct interaction via its N-terminal clathrin-binding domain | Homo sapiens | 30136 | - |
| additional information | both PI3K-C2alpha and PI3K-C2beta are co-localized with clathrin-coated pits and vesicles. PI3K-C2beta, but not PI3K-C2alpha, is highly co-localized with actin filament-associated clathrin-coated structures and required for actin filament formation at the clathrin-coated structures | Homo sapiens | - |
- |
| vesicle | - |
Homo sapiens | 31982 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O00443 | - |
- |
| Homo sapiens | O00750 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| umbilical vein endothelial cell | HUVEC | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| class II phosphoinositide 3-kinases PI3K-C2alpha | - |
Homo sapiens |
| class II PI3K | - |
Homo sapiens |
| PI3K-C2alpha | - |
Homo sapiens |
| PI3K-C2beta | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | ITSN1 knockdown dramatically reduces the localization of PI3K-C2beta at actin patches and at clathrin-coated structures | Homo sapiens |
| malfunction | knockdown of PI3K-C2alpha inhibits internalization of cell surface molecules | Homo sapiens |
| metabolism | knockdown studies of endogenous PI3K isoforms and clathrin heavy chain (CHC) mediated by small interfering RNA (siRNA) demonstrate that the class II PI3K PI3K-C2alpha and PI3K-C2beta, but not the class I or III PI3K, are required for pinocytosis, based on an evaluation of fluorescein-5-isothiocyanate (FITC)-dextran uptake in endothelial cells. Pinocytosis is partially dependent on both clathrin and dynamin, and both PI3K-C2alpha and PI3K-C2beta are required for clathrin-mediated, but not clathrin-nonmediated, FITC-dextran uptake at the step leading up to its delivery to early endosomes. PI3K-C2alpha and PI3K-C2beta play differential, indispensable roles in clathrin-mediated pinocytosis | Homo sapiens |
| physiological function | class II phosphoinositide 3-kinases PI3K-C2alpha and PI3K-C2beta differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells. Pinocytosis is partially dependent on both clathrin and dynamin, and both PI3K-C2alpha and PI3K-C2beta are required for clathrin-mediated, but not clathrin-nonmediated, FITC-dextran uptake at the step leading up to its delivery to early endosomes. PI3K-C2alpha and PI3K-C2beta play differential, indispensable roles in clathrin-mediated pinocytosis | Homo sapiens |
| physiological function | class II phosphoinositide 3-kinases PI3K-C2alpha and PI3K-C2beta differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells. Pinocytosis is partially dependent on both clathrin and dynamin, and both PI3K-C2alpha and PI3K-C2beta are required for clathrin-mediated, but not clathrin-nonmediated, FITC-dextran uptake at the step leading up to its delivery to early endosomes. PI3K-C2alpha and PI3K-C2beta play differential, indispensable roles in clathrin-mediated pinocytosis. Endogenous ITSN1 is required for the formation of actin patches and recruitment of PI3K-C2beta to the clathrin-coated structures. mCherry-C2beta overexpression stimulates pinocytosis through the mechanisms involving GFP-ITSN1-mediated recruitment of mCherry-C2beta | Homo sapiens |
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