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Literature summary for 2.7.1.154 extracted from

  • Nigorikawa, K.; Hazeki, K.; Guo, Y.; Hazeki, O.
    Involvement of class II phosphoinositide 3-kinase ?-isoform in antigen-induced degranulation in RBL-2H3 cells. (2014), PLoS ONE, 9, e111698.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.154

Cloned(Commentary)

Cloned (Comment) Organism
real-time PCR analysis of PI3K-C2alpha and PI3K-C2beta in cells transfected with the control or shRNA expression vector NPY-mRFP and EGFP-PI3K-C2alpha are transfected into RBL-2H3 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
yes knockout of PI3K-C2alpha and PI3K-C2beta by shRNA expression Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Wortmannin a pan-PI3K inhibitor Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
vesicle existence of PI3K-C2alpha and 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate on CD63-positive large vesicles in FceRI-stimulated RBL-2H3 cells Rattus norvegicus 31982
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Organism

Organism UniProt Comment Textmining
Rattus norvegicus
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
RBL-2H3 cell
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 Rattus norvegicus
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Synonyms

Synonyms Comment Organism
class II phosphoinositide 3-kinase alpha
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 Rattus norvegicus
PI3K-C2alpha
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 Rattus norvegicus
PI3K-C2beta
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 Rattus norvegicus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00042
-
 isozyme PI3K-C2alpha, pH and temperature not specified in the publication Rattus norvegicus Wortmannin

General Information

General Information Comment Organism
malfunction PI3K-C2alpha knockdown decreases the FceRI-triggered release of a lysosomal enzyme, namely beta-hexosaminidase, protein levels of PI3K-C2beta are not significantly affected by the shRNAs. siRNA against PI3K-C2beta impairs the FceRI-induced increase in intracellular Ca2+ and degranulation. Release of NPY-mRFP, a reporter of the regulated exocytosis of mast cells is increased by PI3K-C2alpha overexpression and is impaired in the PI3K-C2alpha-deficient cells Rattus norvegicus
metabolism involvement of PI3K-C2alpha in the antigen-induced degranulation pathway Rattus norvegicus
physiological function PI3K-C2beta regulates the FceRI-stimulated activation of the KCa3.1 channel and the FceRI-induced Ca2+ influx and degranulation in BMMCs. PI3K-C2alpha regulates the FceRI-triggered degranulation by a mechanism different from that induced by PI3K-C2beta. PI3KC2alpha and PI3K-C2beta share different roles in the FceRI-regulated degranulation pathway Rattus norvegicus