Cloned (Comment) | Organism |
---|---|
isozyme Ipk2alpha, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain | Arabidopsis thaliana |
isozyme Ipk2beta, location on chromosome 5, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant isozyme in Escherichia coli with or without GST-fusion tag, functional complementation of an enzyme-deficient yeast strain | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
D100A | isozyme IPK2beta, site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
D98A | isozyme IPK2alpha, site-directed mutagenesis, inactive mutant | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
1D-myo-inositol 1,4,5-trisphosphate | pH 7.5, 37°C, recombinant isozyme Ipk2alpha | Arabidopsis thaliana | |
0.016 | - |
1D-myo-inositol 1,3,4,5-tetrakisphosphate | pH 7.5, 37°C, recombinant isozyme Ipk2alpha | Arabidopsis thaliana | |
0.032 | - |
1D-myo-inositol 1,4,5,6-tetrakisphosphate | pH 7.5, 37°C, recombinant isozyme Ipk2alpha | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Arabidopsis thaliana | |
Mg2+ | - |
Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FLT2 | isozyme Ipk2beta; isozyme Ipk2beta | - |
Arabidopsis thaliana | Q9LY23 | isozyme Ipk2alpha; isozyme Ipk2alpha | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-fusion isozymes from Escherichia coli by glutathione affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | ubiquitous expression of isoyzme Ipk2beta | Arabidopsis thaliana | - |
additional information | ubiquitous expression of isozyme Ipk2alpha | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,2,3,4,6-pentakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,2,3,4,5,6-hexakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
? | |
additional information | substrate specificity, no formation of 1D-myo-inositol 1,3,4,5,6-pentakisphosphate from 1D-myo-inositol 1,4,5-trisphosphate | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IP3/IP4 6-/3-kinase | - |
Arabidopsis thaliana |
Ipk2 | - |
Arabidopsis thaliana |
More | the enzyme belongs to the IPK family of enzymes | Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
1 min, inactivation, purified recombinant isozyme | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |