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Literature summary for 2.7.1.151 extracted from
- In Saccharomyces cerevisiae, the inositol polyphosphate kinase activity of Kcs1p is required for resistance to salt stress, cell wall integrity, and vacuolar morphogenesis (2002), J. Biol. Chem., 277, 23755-23763.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene KCS1, subcloning in Escherichia coli strain XL1-B, overexpression in strain arg82DELTA, expression in strain 12S16cpep4D | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| L794A/L801A | site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, phenotype overview | Saccharomyces cerevisiae |
| L794A/L801A/L857A/L864A | site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, the mutant strain shows compromised cell wall integrity, phenotype overview | Saccharomyces cerevisiae |
| L857A/L864A | site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, phenotype overview | Saccharomyces cerevisiae |
| additional information | construction of a knockout mutant strain from strain BY4709, the mutant cells contain a fragmented vacuolar compartment, cell growth and cell wall integrity are perturbed, and response to sal stress is altered, a metabolic block in arg82DELTA cells results in the same phenotype, overexpression of the enzyme can partially bypass the metabolic block, overview, expression of full length enzyme and enzyme mutants in the knockout strain, phenotypes overview | Saccharomyces cerevisiae |
| S887A/L888A/L889A | site-directed mutagenesis, expression in the knockout mutant strain results in activity similar to the wild-type enzyme, the mutant strain shows compromised cell wall integrity, phenotype overview | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | Saccharomyces cerevisiae | - |
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
ir | |
| ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | Saccharomyces cerevisiae | - |
ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
ir | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Saccharomyces cerevisiae | - |
ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
ir | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | Saccharomyces cerevisiae | - |
ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
ir | |
| additional information | Saccharomyces cerevisiae | the enzyme is required for resistance to salt stress, cell wall integrity, and vascuolar morphogenesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from strain expression in strain 12S16cpep4D by nickel affinity chromatography | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amount of different inositol phosphates in the knockout mutant strain with or without expression of enzyme and mutants, overview | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
Saccharomyces cerevisiae | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
ir | |
| ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
Saccharomyces cerevisiae | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
ir | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Saccharomyces cerevisiae | ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate | - |
ir | |
| ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Saccharomyces cerevisiae | ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
ir | |
| additional information | the enzyme is required for resistance to salt stress, cell wall integrity, and vascuolar morphogenesis | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| inositol polyphosphate kinase | - |
Saccharomyces cerevisiae |
| Kcs1p | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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