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Literature summary for 2.7.1.150 extracted from

  • Cobley, D.; Halova, L.; Schauries, M.; Kaczmarek, A.; Franz-Wachtel, M.; Du, W.; Krug, K.; Macek, B.; Petersen, J.
    Ste12/Fab1 phosphatidylinositol-3-phosphate 5-kinase is required for nitrogen-regulated mitotic commitment and cell size control (2017), PLoS ONE, 12, e0172740 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.150

Cloned(Commentary)

Cloned (Comment) Organism
gene ste12, DNA and amino acid sequence determination and analysis Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
additional information identification of a non-functional ste12PIKFYVE mutant that is unable to invoke the normal advancement of mitotic onset and adjust cell size at division in response to nitrogen stress Schizosaccharomyces pombe
W1037stop gene ste12 next generation sequencing reveals a truncating, premature stop codon at position W1037 of the Ste12PIKFYVE-phosphatidylinositol-3-phosphate 5-kinase. The advancement of cell division is markedly compromised in ste12PIKFYVE. Mutant W1037STOP cells as a consequence of a reduction in the efficiency with which mitosis is advanced by the stress. Thus, ste12PIKFYVE.W1037STOP cells are deficient in their nitrogen stress response. Rapamycin rescues both the inability to advance mitosis and reduce cell size at division, suggesting that Ste12 may acts upstream of TORC1. No significant changes in TORC1 and TORC2 activities are observed in ste12PIKFYVE-W1037STOP mutants. Phenotype, overview Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate Schizosaccharomyces pombe
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 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate Schizosaccharomyces pombe ATCC 24843
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate Schizosaccharomyces pombe 972
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O59722
-
-
Schizosaccharomyces pombe 972 O59722
-
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Schizosaccharomyces pombe ATCC 24843 O59722
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Schizosaccharomyces pombe ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Schizosaccharomyces pombe ATCC 24843 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 Schizosaccharomyces pombe 972 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
Fab1
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 Schizosaccharomyces pombe
Phosphatidylinositol-3-phosphate 5-kinase
-
 Schizosaccharomyces pombe
PIKfyve
-
 Schizosaccharomyces pombe
Ste12
-
 Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Schizosaccharomyces pombe

General Information

General Information Comment Organism
malfunction Ste12PIKFYVE-deficient mutants are unable to advance the cell cycle to reduce cell size after a nitrogen downshift to poor nitrogen (proline) growth conditions. Ste12PIKFYVE mutants have enlarged vacuoles (yeast lysosomes), neither a block to autophagy or mutants that independently have enlarged vacuoles has any impact upon nitrogen control of mitotic commitment. The addition of rapamycin to Ste12PIKFYVE-deficient mutants reduces cell size at division to suggest that Ste12PIKFYVE possibly functions upstream of TORC1. Ste12 mutants display increased Torin1 (TOR inhibitor) sensitivity. No major impact on TORC1 or TORC2 activity is observed in the ste12-deficient mutants. Advancement of cell division is markedly compromised in ste12PIKFYVE.W1037STOP cells as a consequence of a reduction in the efficiency with which mitosis is advanced by the stress. Thus, ste12PIKFYVE-W1037STOP cells are deficient in their nitrogen stress response. Rapamycin rescues both the inability to advance mitosis and reduce cell size at division, suggesting that Ste12 may acts upstream of TORC1. ste12PIKFYVE-W1037STOP autophagy defect is unlikely to account for the inability to control size after nitrogen stress Schizosaccharomyces pombe
physiological function PIKFYVE is a 1-phosphatidylinositol-3-phosphate 5-kinase that is required for the production of a signalling phospholipid required for vacuole functions and endosome dynamics, phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2). PI(3,5)P2 is reported to be a positive regulator of TORC1 activity on the yeast vacuole, that is required for TORC1 inhibition of autophagy under nutrient-rich conditions. PIKFYVE also regulates cell type-specific activation and localization of mTORC1 in 3T3-L1 adipocytes. In humans, mutations predicted to lead to minor changes in PI(3,5)P2 levels are associated with severe neurological diseases and are implicated in the invasive behaviour of cancer cells. Ste12/Fab1 phosphatidylinositol-3-phosphate 5-kinase is required for nitrogen-regulated mitotic commitment and cell size control. Ste12PIKFYVE is required for nitrogen-stress mediated advancement of mitosis to reduce cell size at division Schizosaccharomyces pombe