Cloned (Comment) | Organism |
---|---|
gene PIKfyve, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana plants | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knockdown of FAB1A/B expression | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
YM201636 | a selective FAB1/PIKfyve inhibitor | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | the enzyme predominantly localizes on the Sorting Nexin1 (SNX1)-residing late endosomes, the SNX1-positive endosomes | Arabidopsis thaliana | 5768 | - |
membrane | - |
Arabidopsis thaliana | 16020 | - |
microtubule | cortical | Arabidopsis thaliana | 5874 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | Arabidopsis thaliana | - |
ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate | - |
? | |
additional information | Arabidopsis thaliana | TLC measurement of phospholipids in wild-type and FAB1A-GFP-expressing plants, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9LUM0 | gene PIKfyve | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | cortical cell | Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate | - |
Arabidopsis thaliana | ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate | - |
? | |
additional information | TLC measurement of phospholipids in wild-type and FAB1A-GFP-expressing plants, overview | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Fab1 | - |
Arabidopsis thaliana |
FAB1/FYVE finger-containing phosphoinositide kinase | - |
Arabidopsis thaliana |
formation of aploid and binucleate cells1/FYVE finger-containing phosphoinositide kinase | - |
Arabidopsis thaliana |
phosphatidylinositol 3-phosphate 5-kinase | - |
Arabidopsis thaliana |
PIKfyve | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | a loss of enzyme function causes the release of late endosomal proteins, Ara7, and SNX1 from the endosome membrane. Downregulation of FAB1A/B or YM201636 inhibitor treatment affects the auxin distribution and and alteration of PIN2 localization in root cells. FAB1 knockdown causes relocation of the basal polarity of PIN2 in young root cortical cells | Arabidopsis thaliana |
physiological function | the enzyme mediates endosome maturation to establish endosome-cortical microtubule interaction in Arabidopsis thaliana. The enzyme and its product, 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate, are essential for the maturation process of endosomes to mediate cortical microtubule association of endosomes, thereby controlling proper PIN-FORMED protein trafficking in young cortical and stele cells of root. The enzyme and its product mediate the late endosome maturation by recruiting endosomal effector molecules, Ara7 and SNX1, onto endosomes to establish endosome-cortical microtubule interaction | Arabidopsis thaliana |