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Literature summary for 2.7.1.150 extracted from

  • Hirano, T.; Munnik, T.; Sato, M.H.
    Phosphatidylinositol 3-phosphate 5-kinase, FAB1/PIKfyve kinase mediates endosome maturation to establish endosome-cortical microtubule interaction in Arabidopsis (2015), Plant Physiol., 169, 1961-1974.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene PIKfyve, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana plants Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information knockdown of FAB1A/B expression Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
YM201636 a selective FAB1/PIKfyve inhibitor Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
endosome the enzyme predominantly localizes on the Sorting Nexin1 (SNX1)-residing late endosomes, the SNX1-positive endosomes Arabidopsis thaliana 5768
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membrane
-
Arabidopsis thaliana 16020
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microtubule cortical Arabidopsis thaliana 5874
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate Arabidopsis thaliana
-
ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
?
additional information Arabidopsis thaliana TLC measurement of phospholipids in wild-type and FAB1A-GFP-expressing plants, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9LUM0 gene PIKfyve
-

Source Tissue

Source Tissue Comment Organism Textmining
root cortical cell Arabidopsis thaliana
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seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
Arabidopsis thaliana ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
?
additional information TLC measurement of phospholipids in wild-type and FAB1A-GFP-expressing plants, overview Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
Fab1
-
Arabidopsis thaliana
FAB1/FYVE finger-containing phosphoinositide kinase
-
Arabidopsis thaliana
formation of aploid and binucleate cells1/FYVE finger-containing phosphoinositide kinase
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Arabidopsis thaliana
phosphatidylinositol 3-phosphate 5-kinase
-
Arabidopsis thaliana
PIKfyve
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction a loss of enzyme function causes the release of late endosomal proteins, Ara7, and SNX1 from the endosome membrane. Downregulation of FAB1A/B or YM201636 inhibitor treatment affects the auxin distribution and and alteration of PIN2 localization in root cells. FAB1 knockdown causes relocation of the basal polarity of PIN2 in young root cortical cells Arabidopsis thaliana
physiological function the enzyme mediates endosome maturation to establish endosome-cortical microtubule interaction in Arabidopsis thaliana. The enzyme and its product, 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate, are essential for the maturation process of endosomes to mediate cortical microtubule association of endosomes, thereby controlling proper PIN-FORMED protein trafficking in young cortical and stele cells of root. The enzyme and its product mediate the late endosome maturation by recruiting endosomal effector molecules, Ara7 and SNX1, onto endosomes to establish endosome-cortical microtubule interaction Arabidopsis thaliana