Literature summary for 2.7.1.15 extracted from

Gatreddi, S.; Pillalamarri, V.; Vasudevan, D.; Addlagatta, A.; Qureshi, I.A.
Unraveling structural insights of ribokinase from Leishmania donovani (2019), Int. J. Biol. Macromol., 136, 253-265 .

Search for more literature for 2.7.1.15

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, the crystal structure is determined in the apo and complex forms with several nucleotides (ATP, AMPPCP and ADP) in the presence of Na+ ion	Leishmania donovani

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	-	Leishmania donovani

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	the enzyme is dependent on monovalent as well as divalent cations for its catalytic activity. When magnesium is substituted with Co2+ and Mn2+ in the reaction mixture, the ribokinase activity got reduced to 60% and 45% as compared to that with Mg2+	Leishmania donovani
K+	the enzyme is dependent on monovalent as well as divalent cations for its catalytic activity. Among the monovalent cations, K+ shows highest activating effect. The activity drops to 90% and 45% with Cs+ and Na+, respectively, in comparison to K+	Leishmania donovani
Mg2+	the enzyme is dependent on monovalent as well as divalent cations for its catalytic activity. When magnesium is substituted with Co2+ and Mn2+ in the reaction mixture, the ribokinase activity got reduced to 60% and 45% as compared to that with Mg2+	Leishmania donovani
Mn2+	the enzyme is dependent on monovalent as well as divalent cations for its catalytic activity. When magnesium is substituted with Co2+ and Mn2+ in the reaction mixture, the ribokinase activity got reduced to 60% and 45% as compared to that with Mg2+	Leishmania donovani

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + D-ribose	Leishmania donovani	-	ADP + D-ribose 5-phosphate	-	?
ATP + D-ribose	Leishmania donovani BPK282A1	-	ADP + D-ribose 5-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	E9BIX7	-	-
Leishmania donovani BPK282A1	E9BIX7	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Leishmania donovani

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-ribose	-	Leishmania donovani	ADP + D-ribose 5-phosphate	-	?
ATP + D-ribose	-	Leishmania donovani BPK282A1	ADP + D-ribose 5-phosphate	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Leishmania donovani

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Leishmania donovani

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Release 2023.1 (January 2023)