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Literature summary for 2.7.1.15 extracted from
- Ribokinase from Leishmania donovani purification, characterization and X-ray crystallographic analysis (2018), Acta Crystallogr. Sect. F, 74, 99-104 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 (DE3) cells | Leishmania donovani |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals obtained by the hanging-drop vapour-diffusion method at 23°C diffract to 1.95 A resolution and belong to the hexagonal space group P61, with unit-cell parameters a = b = 100.25, c = 126.77 A | Leishmania donovani |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.116 | - |
ATP | pH 8.5, 25°C | Leishmania donovani |  |
| 0.296 | - |
D-ribose | pH 8.5, 25°C | Leishmania donovani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
SDS-PAGE | Leishmania donovani |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose | Leishmania donovani | the enzyme catalyzes the first step of ribose metabolism | ADP + D-ribose 5-phosphate | - |
? | |
| ATP + D-ribose | Leishmania donovani BPK282A1 | the enzyme catalyzes the first step of ribose metabolism | ADP + D-ribose 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | E9BIX7 | - |
- |
| Leishmania donovani BPK282A1 | E9BIX7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-affinity and gel-filtration chromatography | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-ribose | - |
Leishmania donovani | ADP + D-ribose 5-phosphate | - |
? | |
| ATP + D-ribose | the enzyme catalyzes the first step of ribose metabolism | Leishmania donovani | ADP + D-ribose 5-phosphate | - |
? | |
| ATP + D-ribose | - |
Leishmania donovani BPK282A1 | ADP + D-ribose 5-phosphate | - |
? | |
| ATP + D-ribose | the enzyme catalyzes the first step of ribose metabolism | Leishmania donovani BPK282A1 | ADP + D-ribose 5-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Leishmania donovani |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Leishmania donovani |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 44 | - |
Tm-value | Leishmania donovani |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.15 | - |
ATP | pH 8.5, 25°C | Leishmania donovani | |
| 2.28 | - |
D-ribose | pH 8.5, 25°C | Leishmania donovani | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Leishmania donovani |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the enzyme catalyzes the first step of ribose metabolism | Leishmania donovani |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.7 | - |
D-ribose | pH 8.5, 25°C | Leishmania donovani | |
| 18.5 | - |
ATP | pH 8.5, 25°C | Leishmania donovani | |
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