Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged isozymes and of HA-tagged isozymes in HEK-293 cells | Homo sapiens |
recombinant expression of Myc-tagged isozymes, co-expression of the wild-type PIP4Kalpha isozyme suppresses ubiquitination of isozyme PIP4Kbeta | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isozymes PIPKalpha and PIP4Kbeta inactivation by specific RNAi. Overexpression of PIP4Kalpha leads to a dramatic increase in PIP4K activity, that is not present when wild-type PIP4Kbeta kinase is expressed | Homo sapiens |
additional information | knockdown of both isozymes, enzyme activity associated with kinase-inactive Myc-PIP4Kbeta is reduced to 24% compared with that associated with the active Myc-PIP4Kbeta | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | PIPKIIbeta and PIPKIIalpha | Rattus norvegicus | 5829 | - |
nucleus | isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha | Homo sapiens | 5634 | - |
nucleus | isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha | Rattus norvegicus | 5634 | - |
plasma membrane | - |
Homo sapiens | 5886 | - |
plasma membrane | - |
Rattus norvegicus | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | required | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
2 * 49000, recombinant isozyme PIP4Kbeta, SDS-PAGE, and crystal structure of isozyme PIP4Kbeta | Homo sapiens |
97000 | - |
recombinant isozyme PIP4Kbeta | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | Homo sapiens | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | Rattus norvegicus | - |
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
additional information | Homo sapiens | isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha | ? | - |
? | |
additional information | Rattus norvegicus | isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged isozymes by glutathione affinity chromatography, recombinant HA-tagged isozymes by affinity chromatography from HEK-293 cells | Homo sapiens |
recombinant Myc-tagged isozymes by affinity chromatography | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | - |
Homo sapiens | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate | - |
Rattus norvegicus | ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate | - |
? | |
additional information | isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha | Homo sapiens | ? | - |
? | |
additional information | isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 49000, recombinant isozyme PIP4Kbeta, SDS-PAGE, and crystal structure of isozyme PIP4Kbeta | Homo sapiens |
homodimer | crystal structure of isozyme PIP4Kbeta | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
PIP4K | - |
Homo sapiens |
PIP4K | - |
Rattus norvegicus |
PIP4Kalpha | - |
Homo sapiens |
PIP4Kalpha | - |
Rattus norvegicus |
PIP4Kbeta | - |
Homo sapiens |
PIP4Kbeta | - |
Rattus norvegicus |
PtdIns5P-4-kinase | - |
Homo sapiens |
PtdIns5P-4-kinase | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
ATP | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | isozyme PIP4Kalpha affects the ubiquitination of isozyme PIP4Kbeta by the SPOP-CUL3 complex. Isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha, and isozyme PIP4Kalpha has a role in regulating isozyme PIP4Kbeta functions | Homo sapiens |
physiological function | isozyme PIP4Kalpha affects the ubiquitination of isozyme PIP4Kbeta by the SPOP-CUL3 complex. Isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha, and isozyme PIP4Kalpha has a role in regulating isozyme PIP4Kbeta functions | Rattus norvegicus |