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Literature summary for 2.7.1.149 extracted from

  • Bultsma, Y.; Keune, W.J.; Divecha, N.
    PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha (2010), Biochem. J., 430, 223-235.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of GST-tagged isozymes and of HA-tagged isozymes in HEK-293 cells Homo sapiens
recombinant expression of Myc-tagged isozymes, co-expression of the wild-type PIP4Kalpha isozyme suppresses ubiquitination of isozyme PIP4Kbeta Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information isozymes PIPKalpha and PIP4Kbeta inactivation by specific RNAi. Overexpression of PIP4Kalpha leads to a dramatic increase in PIP4K activity, that is not present when wild-type PIP4Kbeta kinase is expressed Homo sapiens
additional information knockdown of both isozymes, enzyme activity associated with kinase-inactive Myc-PIP4Kbeta is reduced to 24% compared with that associated with the active Myc-PIP4Kbeta Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
cytosol PIPKIIbeta and PIPKIIalpha Rattus norvegicus 5829
-
nucleus isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha Homo sapiens 5634
-
nucleus isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha Rattus norvegicus 5634
-
plasma membrane
-
Homo sapiens 5886
-
plasma membrane
-
Rattus norvegicus 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens
Mg2+ required Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, recombinant isozyme PIP4Kbeta, SDS-PAGE, and crystal structure of isozyme PIP4Kbeta Homo sapiens
97000
-
recombinant isozyme PIP4Kbeta Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate Rattus norvegicus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
additional information Homo sapiens isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha ?
-
?
additional information Rattus norvegicus isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged isozymes by glutathione affinity chromatography, recombinant HA-tagged isozymes by affinity chromatography from HEK-293 cells Homo sapiens
recombinant Myc-tagged isozymes by affinity chromatography Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate
-
Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
?
additional information isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha Homo sapiens ?
-
?
additional information isozyme PIP4Kbeta interacts in vitro and in vivo with the PIP4Kalpha isoform. PIP4Kbeta has 2000fold less activity towards 1-phosphatidyl-1D-myo-inositol 5-phosphate compared with PIP4Kalpha, the majority of enzyme activity associated with PIP4Kbeta comes from its interaction with PIP4Kalpha Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 49000, recombinant isozyme PIP4Kbeta, SDS-PAGE, and crystal structure of isozyme PIP4Kbeta Homo sapiens
homodimer crystal structure of isozyme PIP4Kbeta Rattus norvegicus

Synonyms

Synonyms Comment Organism
PIP4K
-
Homo sapiens
PIP4K
-
Rattus norvegicus
PIP4Kalpha
-
Homo sapiens
PIP4Kalpha
-
Rattus norvegicus
PIP4Kbeta
-
Homo sapiens
PIP4Kbeta
-
Rattus norvegicus
PtdIns5P-4-kinase
-
Homo sapiens
PtdIns5P-4-kinase
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens
ATP
-
Rattus norvegicus

General Information

General Information Comment Organism
physiological function isozyme PIP4Kalpha affects the ubiquitination of isozyme PIP4Kbeta by the SPOP-CUL3 complex. Isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha, and isozyme PIP4Kalpha has a role in regulating isozyme PIP4Kbeta functions Homo sapiens
physiological function isozyme PIP4Kalpha affects the ubiquitination of isozyme PIP4Kbeta by the SPOP-CUL3 complex. Isozyme PIP4Kbeta can modulate the nuclear localization of isozyme PIP4Kalpha, and isozyme PIP4Kalpha has a role in regulating isozyme PIP4Kbeta functions Rattus norvegicus