Literature summary for 2.7.1.148 extracted from

Kadian, K.; Vijay, S.; Gupta, Y.; Rawal, R.; Singh, J.; Anvikar, A.; Pande, V.; Sharma, A.
Structural modeling identifies Plasmodium vivax 4-diphosphocytidyl-2C-methyl-d-erythritol kinase (IspE) as a plausible new antimalarial drug target (2018), Parasitol. Int., 67, 375-385 .

Search for more literature for 2.7.1.148

Cloned(Commentary)

Cloned (Comment)	Organism
gene ispE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, genotyping. Multiple sequence analysis (MSA) of deduced amino acid sequences of PvIspE gene reveals that PvIspE amino acid sequences are regionally conserved as they show 100% similarity among all Indian isolates from a particular region. Specific mutations are found in different regions	Plasmodium vivax

Cloned (Comment)

Organism

gene ispE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, genotyping. Multiple sequence analysis (MSA) of deduced amino acid sequences of PvIspE gene reveals that PvIspE amino acid sequences are regionally conserved as they show 100% similarity among all Indian isolates from a particular region. Specific mutations are found in different regions

Plasmodium vivax

Protein Variants

Protein Variants	Comment	Organism
A382T	naturally occuring mutation, neutral	Plasmodium vivax
G402R	naturally occuring mutation, neutral	Plasmodium vivax
K492T	naturally occuring mutation, neutral	Plasmodium vivax
L405Q	naturally occuring mutation, neutral	Plasmodium vivax
additional information	two non-synonymous mutations at codons 382 (A->T) and 492 (K->T) are found in all the isolates of Delhi, Chennai, Nadiad, Sonapur Goa, and Mangaluru. Apart from these, two more mutations are observed in all the isolates of Goa and Mangaluru namely one substitution at codon 351(T->M) and another synonymous mutation at codon 344 (S) TCT->TCC. In Rourkela which is one of the highly malaria endemic and isolated tribal regions of India, four non-synonymous mutations at codons 336 (R->S), 351 (T->M), 402 (G->R) and 405 (L->Q) are detected in all isolates. Domain architecture and mutation mapping, overview	Plasmodium vivax
R336S	naturally occuring mutation, neutral	Plasmodium vivax
T351M	naturally occuring mutation, neutral	Plasmodium vivax

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Plasmodium vivax

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	Plasmodium vivax	-	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	Plasmodium vivax Salvador I	-	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium vivax	A5KAU8	clinical blood samples of Plasmodium vivax positive patients collected from seven malaria endemic geographical regions of India with entirely different topography viz. Delhi (New Delhi), Nadiad (Gujarat), Rourkela (Orissa), Goa (Goa), Mangaluru (Karnataka), Chennai (Tamil Nadu) and Sonapur (Assam)	-
Plasmodium vivax Salvador I	A5KAU8	clinical blood samples of Plasmodium vivax positive patients collected from seven malaria endemic geographical regions of India with entirely different topography viz. Delhi (New Delhi), Nadiad (Gujarat), Rourkela (Orissa), Goa (Goa), Mangaluru (Karnataka), Chennai (Tamil Nadu) and Sonapur (Assam)	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	Plasmodium vivax	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	Plasmodium vivax Salvador I	ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol	-	?

Synonyms

Synonyms	Comment	Organism
IspE	-	Plasmodium vivax
PvIspE	-	Plasmodium vivax

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Plasmodium vivax

General Information

General Information	Comment	Organism
additional information	three-dimensional structure and active site structure predictions. The active site of PvIspE consists of total 32 amino acid residues, 16 amino acid residues involved in ATP binding (Y225, P226, D230, N231, I232, K258, I262, F263, S264, G265, G267, G268, G269, N272, D304, S349) and 16 amino acid residues interacts with the substrate CDM (K136, N138, L141, H150, N151, M157, S270, G302, S303, D304, K319, S349, R350, Y353, L413, G444)	Plasmodium vivax