Application | Comment | Organism |
---|---|---|
drug development | enzyme is a target for antimicrobial drug development | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
gene ychB, expression of the selenomethionine-labeled enzyme in Escherichia coli strain B834(DE3) | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 20°C, 0.002 ml protein solution containing 2.2 mg/ml protein, 20 ml Tris-HCl, pH 8.0, and 50 mM NaCl, are mixed with 0.001 ml mother liquor containing 33 mM Tris-HCl, pH 8.5, 67 mM sodium acetate, 13% isopropanol, 8% butanol, and 13% PEG 4000, cryoprotection at -173°C in 30% glycerol, X-ray diffraction structure determination and analysis at 1.7 A resolution | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding site structure, S95, D125, and G94 are important | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | Thermus thermophilus | the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant selenomethionine-labeled enzyme from Escherichia coli strain B834(DE3) by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography, gel filtration, and ultrafiltration | Thermus thermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | reaction mechanism involves K8 and D125 | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview | Thermus thermophilus | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | i.e. CDP-ME, the cytosine group plays an essential role in substrate recognition by the enzyme | Thermus thermophilus | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | the enzyme is essential in the nonmevalonate pathway of isopentenyl diphosphate and dimethylallyl diphosphate biosynthesis, pathway overview | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | i.e. CDP-ME, the cytosine group plays an essential role in substrate recognition by the enzyme | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
additional information | no activity with 4-(adenine 5'-phosphate)-2-C-methyl-D-erythritol and 4-(uridine 5'-phosphate)-2-C-methyl-D-erythritol | Thermus thermophilus | ? | - |
? | |
additional information | no activity with 4-(adenine 5'-phosphate)-2-C-methyl-D-erythritol and 4-(uridine 5'-phosphate)-2-C-methyl-D-erythritol | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase | - |
Thermus thermophilus |
CDP-ME kinase | - |
Thermus thermophilus |
CMK | - |
Thermus thermophilus |
More | enzyme belongs to the GHMK super family | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding site structure, N58 and K83 are important | Thermus thermophilus |