Protein Variants | Comment | Organism |
---|---|---|
E279D | mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ | Thermococcus litoralis |
E279L | mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ | Thermococcus litoralis |
E279Q | mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ | Thermococcus litoralis |
E308Q | mutation increases the KM value for MgADP-, mutant is activated by free Mg2+ | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
ADP | wild-type, pH 7.8, 40°C | Thermococcus litoralis | |
0.034 | - |
ADP | mutant E279L, pH 7.8, 40°C | Thermococcus litoralis | |
0.066 | - |
ADP | mutant E279Q, pH 7.8, 40°C | Thermococcus litoralis | |
0.27 | - |
ADP | mutant E279D, pH 7.8, 40°C | Thermococcus litoralis | |
0.28 | - |
D-glucose | wild-type, pH 7.8, 40°C | Thermococcus litoralis | |
0.39 | - |
D-glucose | mutant E308Q, pH 7.8, 40°C | Thermococcus litoralis | |
1.46 | - |
ADP | mutant E308Q, pH 7.8, 40°C | Thermococcus litoralis | |
8 | - |
D-glucose | mutant E279D, pH 7.8, 40°C | Thermococcus litoralis | |
8.9 | - |
D-glucose | mutant E279Q, pH 7.8, 40°C | Thermococcus litoralis | |
109 | - |
D-glucose | mutant E279L, pH 7.8, 40°C | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | Q7M537 | - |
- |
Thermococcus litoralis DSM 5473 | Q7M537 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-glucose | - |
Thermococcus litoralis | AMP + D-glucose 6-phosphate | - |
? | |
ADP + D-glucose | - |
Thermococcus litoralis DSM 5473 | AMP + D-glucose 6-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
ADP | mutant E279L, pH 7.8, 40°C | Thermococcus litoralis | |
0.71 | - |
ADP | mutant E279Q, pH 7.8, 40°C | Thermococcus litoralis | |
1.52 | - |
ADP | mutant E279D, pH 7.8, 40°C | Thermococcus litoralis | |
4.3 | - |
ADP | mutant E308Q, pH 7.8, 40°C | Thermococcus litoralis | |
58 | - |
ADP | wild-type, pH 7.8, 40°C | Thermococcus litoralis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
D-glucose | mutant E279L, pH 7.8, 40°C | Thermococcus litoralis | |
0.079 | - |
D-glucose | mutant E279Q, pH 7.8, 40°C | Thermococcus litoralis | |
0.19 | - |
D-glucose | mutant E279D, pH 7.8, 40°C | Thermococcus litoralis | |
2.95 | - |
ADP | mutant E308Q, pH 7.8, 40°C | Thermococcus litoralis | |
5.63 | - |
ADP | mutant E279D, pH 7.8, 40°C | Thermococcus litoralis | |
10.7 | - |
ADP | mutant E279Q, pH 7.8, 40°C | Thermococcus litoralis | |
11 | - |
D-glucose | mutant E308Q, pH 7.8, 40°C | Thermococcus litoralis | |
16.4 | - |
ADP | mutant E279L, pH 7.8, 40°C | Thermococcus litoralis | |
207 | - |
D-glucose | wild-type, pH 7.8, 40°C | Thermococcus litoralis | |
4140 | - |
ADP | wild-type, pH 7.8, 40°C | Thermococcus litoralis |