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Literature summary for 2.7.1.147 extracted from
- Cloning and biochemical characterization of a novel mouse ADP-dependent glucokinase (2004), Biochem. Biophys. Res. Commun., 315, 652-658.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by D-glucose 6-phosphate, no or nearly no inhibition by L-glucose, glucose 1-phosphate, fructose 1-phosphate, 2,3-bisphosphoglycerate, succinate, proline, N-acetylglucosamine, diphosphate, mannose, galactose, fructose, ribose, NH4Cl, IMP, glucosamine, inositol, and phosphocreatine | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain DH5alpha | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | excess Mg2+ is inhibitory, 55% inhibition at 55 mM, 41% at 15 mM, and 22% at 10 mM | Mus musculus |  |
| additional information | no inhibition by D-glucose 6-phosphate, no or nearly no inhibition by L-glucose, glucose 1-phosphate, fructose 1-phosphate, 2,3-bisphosphoglycerate, succinate, proline, N-acetylglucosamine, diphosphate, mannose, galactose, fructose, ribose, NH4Cl, IMP, glucosamine, inositol, phosphocreatine, ATP, GTP, CTP, GDP, UTP, TTP, NADP+, oxaloacetate, TDP, CDP, UDP, palmitate, D-glucose 1,6-bisphosphate, and several other compounds, overview | Mus musculus | |
| NaCl | 84% of maximal activity at 0.5 M, 48% of maximal activity at 1 M | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Mus musculus | |
| 0.028 | - |
ADP | at 0.8 mM and an ADP:Mg2+ ratio of 1:1, pH 7.0, 37°C | Mus musculus | |
| 0.096 | - |
D-glucose | at below 0.35 mM, pH 7.0, 37°C | Mus musculus | |
| 20 | - |
GDP | pH 7.0, 37°C | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | required at 0.5 M for full enzyme activity | Mus musculus | |
| Mg2+ | best at ADP-Mg2+ ratio of 1:1, excess Mg2+ is inhibitory | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51500 | - |
1 * 51500, recombinant enzyme, SDS-PAGE | Mus musculus |
| 60300 | - |
recombinant enzyme, gel filtration | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + D-glucose | Mus musculus | - |
AMP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q8VDL4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain DH5alpha by nickel affinity and ion exchange chromatography, to homogeneity | Mus musculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.7 | - |
purified recombinant enzyme, pH 7.0, 37°C, substrate D-glucose | Mus musculus |
| 17 | - |
purified recombinant enzyme, pH 6.0, 37°C, substrate D-glucose | Mus musculus |
| 29.3 | - |
purified recombinant enzyme, pH 9.0, 37°C, substrate D-glucose | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + D-fructose | 10% of the activity with D-glucose | Mus musculus | AMP + D-fructose 6-phosphate | - |
? | |
| ADP + D-glucose | - |
Mus musculus | AMP + D-glucose 6-phosphate | - |
? | |
| ADP + D-glucose | best substrate concentration is 0.35 mM glucose, high concentrations are inhibitory | Mus musculus | AMP + D-glucose 6-phosphate | - |
? | |
| ADP + D-mannose | 20% of the activity with D-glucose | Mus musculus | AMP + D-mannose 6-phosphate | - |
? | |
| CDP + D-glucose | 12% of the activity with ADP | Mus musculus | CMP + D-glucose 6-phosphate | - |
? | |
| GDP + D-glucose | 55% of the activity with ADP | Mus musculus | GMP + D-glucose 6-phosphate | - |
? | |
| additional information | no activity with ATP, GTP, CTP, UTP, TTP, diphosphate, polyphosphate, TDP, UDP, acetyl-phosphate, phosphoarginine, carbamoyl phosphate, phosphocreatine, and phosphoenolpyruvate as phosphoryl donors | Mus musculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51500, recombinant enzyme, SDS-PAGE | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADP-dependent glucokinase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.5 | bimodal optima at pH 5.8-6.5 and at 8.8-9.0 | Mus musculus |
| 8.8 | 9 | bimodal optima at pH 5.8-6.5 and at 8.8-9.0 | Mus musculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | ADP-Mg2+ ratio of 1:1 | Mus musculus | |
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