KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ADP | pH 7.6, 50°C | Archaeoglobus fulgidus | |
0.78 | - |
D-glucose | pH 7.6, 50°C | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Archaeoglobus fulgidus | |
Co2+ | - |
Archaeoglobus fulgidus | |
Cu2+ | - |
Archaeoglobus fulgidus | |
Mg2+ | at 4 mM, optimal ratio of ADP:MgCl2 is 1:2 | Archaeoglobus fulgidus | |
Mn2+ | - |
Archaeoglobus fulgidus | |
additional information | divalent cations are required, Mg2+, Mn2+, and Ca2+ are most effective, Cu2+, Zn2+, Ni2+, and Cu2+ can partially substitute | Archaeoglobus fulgidus | |
Ni2+ | - |
Archaeoglobus fulgidus | |
Zn2+ | - |
Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-glucose | Archaeoglobus fulgidus | first step of the Embden-Meyerhoff pathway | AMP + D-glucose 6-phosphate | - |
r | |
ADP + D-glucose | Archaeoglobus fulgidus 7324 | first step of the Embden-Meyerhoff pathway | AMP + D-glucose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Archaeoglobus fulgidus 7324 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
enzyme is not sensitive to oxygen | Archaeoglobus fulgidus |
Purification (Comment) | Organism |
---|---|
600fold to homogeneity by ultracentrifugation, ion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and 2 additional ion exchange chromatographic steps | Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Archaeoglobus fulgidus |
58.9 | - |
purified enzyme | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-glucosamine | the enzyme is highly specific for both substrates | Archaeoglobus fulgidus | AMP + D-glucosamine 6-phosphate | - |
r | |
ADP + D-glucosamine | the enzyme is highly specific for both substrates | Archaeoglobus fulgidus 7324 | AMP + D-glucosamine 6-phosphate | - |
r | |
ADP + D-glucose | first step of the Embden-Meyerhoff pathway | Archaeoglobus fulgidus | AMP + D-glucose 6-phosphate | - |
r | |
ADP + D-glucose | the enzyme is highly specific for both substrates | Archaeoglobus fulgidus | AMP + D-glucose 6-phosphate | - |
r | |
ADP + D-glucose | first step of the Embden-Meyerhoff pathway | Archaeoglobus fulgidus 7324 | AMP + D-glucose 6-phosphate | - |
r | |
ADP + D-glucose | the enzyme is highly specific for both substrates | Archaeoglobus fulgidus 7324 | AMP + D-glucose 6-phosphate | - |
r | |
additional information | 2-deoxyglucose is a poor substrate, no activity with ATP, CDP, UDP, or GDP as phosphate donors, no activity with mannose, fructose, and fructose 6-phosphate as phosphate acceptors | Archaeoglobus fulgidus | ? | - |
? | |
additional information | 2-deoxyglucose is a poor substrate, no activity with ATP, CDP, UDP, or GDP as phosphate donors, no activity with mannose, fructose, and fructose 6-phosphate as phosphate acceptors | Archaeoglobus fulgidus 7324 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
ADP-dependent glucokinase | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
83 | - |
- |
Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable up to | Archaeoglobus fulgidus |
100 | - |
in absence of salt loss of over 80% activity within 30 min, in presence of 1 M ammonium sulfate 70% reaminin g activity after 120 min, in presence of 1 M NaCl 40% remaining activity after 120 min | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | at 2 mM, optimal ratio of ADP:MgCl2 is 1:2 | Archaeoglobus fulgidus |