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Literature summary for 2.7.1.147 extracted from

  • Labes, A.; Schonheit, P.
    ADP-dependent glucokinase from the hyperthermophilic sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324 (2003), Arch. Microbiol., 180, 69-75.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
ADP pH 7.6, 50°C Archaeoglobus fulgidus
0.78
-
D-glucose pH 7.6, 50°C Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Archaeoglobus fulgidus
Co2+
-
Archaeoglobus fulgidus
Cu2+
-
Archaeoglobus fulgidus
Mg2+ at 4 mM, optimal ratio of ADP:MgCl2 is 1:2 Archaeoglobus fulgidus
Mn2+
-
Archaeoglobus fulgidus
additional information divalent cations are required, Mg2+, Mn2+, and Ca2+ are most effective, Cu2+, Zn2+, Ni2+, and Cu2+ can partially substitute Archaeoglobus fulgidus
Ni2+
-
Archaeoglobus fulgidus
Zn2+
-
Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + D-glucose Archaeoglobus fulgidus first step of the Embden-Meyerhoff pathway AMP + D-glucose 6-phosphate
-
r
ADP + D-glucose Archaeoglobus fulgidus 7324 first step of the Embden-Meyerhoff pathway AMP + D-glucose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Archaeoglobus fulgidus 7324
-
-
-

Oxidation Stability

Oxidation Stability Organism
enzyme is not sensitive to oxygen Archaeoglobus fulgidus

Purification (Commentary)

Purification (Comment) Organism
600fold to homogeneity by ultracentrifugation, ion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, hydroxyapatite chromatography, and 2 additional ion exchange chromatographic steps Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Archaeoglobus fulgidus
58.9
-
purified enzyme Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + D-glucosamine the enzyme is highly specific for both substrates Archaeoglobus fulgidus AMP + D-glucosamine 6-phosphate
-
r
ADP + D-glucosamine the enzyme is highly specific for both substrates Archaeoglobus fulgidus 7324 AMP + D-glucosamine 6-phosphate
-
r
ADP + D-glucose first step of the Embden-Meyerhoff pathway Archaeoglobus fulgidus AMP + D-glucose 6-phosphate
-
r
ADP + D-glucose the enzyme is highly specific for both substrates Archaeoglobus fulgidus AMP + D-glucose 6-phosphate
-
r
ADP + D-glucose first step of the Embden-Meyerhoff pathway Archaeoglobus fulgidus 7324 AMP + D-glucose 6-phosphate
-
r
ADP + D-glucose the enzyme is highly specific for both substrates Archaeoglobus fulgidus 7324 AMP + D-glucose 6-phosphate
-
r
additional information 2-deoxyglucose is a poor substrate, no activity with ATP, CDP, UDP, or GDP as phosphate donors, no activity with mannose, fructose, and fructose 6-phosphate as phosphate acceptors Archaeoglobus fulgidus ?
-
?
additional information 2-deoxyglucose is a poor substrate, no activity with ATP, CDP, UDP, or GDP as phosphate donors, no activity with mannose, fructose, and fructose 6-phosphate as phosphate acceptors Archaeoglobus fulgidus 7324 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
ADP-dependent glucokinase
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
83
-
-
Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
stable up to Archaeoglobus fulgidus
100
-
in absence of salt loss of over 80% activity within 30 min, in presence of 1 M ammonium sulfate 70% reaminin g activity after 120 min, in presence of 1 M NaCl 40% remaining activity after 120 min Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
ADP at 2 mM, optimal ratio of ADP:MgCl2 is 1:2 Archaeoglobus fulgidus