Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Thermococcus litoralis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 10 mg/ml protein in 150 mM NaCl, 5 mM Tris-HCl, pH 7.5, is mixed with an equal volume of reservoir solution containing 4 M NaCl, 100 mM Tris-HCl, pH 7.5, 1 week at 25°C, derivatization by soaking for 2 days in 2.85 mM K2PtCl4, 2.8 mM HAuCl4, and 2 mM 4-hydroxymercuribenzoic acid or 0.4 mM 4-hydroxymercuribenzenesulfonic acid, cryoprotection by 20% glycerol in mother liquor, X-ray diffraction structure determination and analysis at 2.6 A resolution | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.56 | - |
D-fructose 6-phosphate | - |
Thermococcus litoralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, recombinant enzyme, SDS-PAGE | Thermococcus litoralis |
125000 | - |
recombinant enzyme, gel filtration | Thermococcus litoralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-fructose 6-phosphate | Thermococcus litoralis | - |
AMP + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | Q977Q3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration | Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
157 | - |
purified recombinant enzyme | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + D-fructose 6-phosphate | - |
Thermococcus litoralis | AMP + D-fructose 1,6-bisphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, recombinant enzyme, SDS-PAGE | Thermococcus litoralis |
Synonyms | Comment | Organism |
---|---|---|
ADP-dependent phosphofructokinase | - |
Thermococcus litoralis |
TLPFK | - |
Thermococcus litoralis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Thermococcus litoralis | |
additional information | no activity with ATP | Thermococcus litoralis |