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Literature summary for 2.7.1.145 extracted from

  • Chen, F.; Zhang, Y.; Daugherty, A.B.; Yang, Z.; Shaw, R.; Dong, M.; Lutz, S.; Benner, S.A.
    Biological phosphorylation of an unnatural base pair (UBP) using a Drosophila melanogaster deoxynucleoside kinase (DmdNK) mutant (2017), PLoS ONE, 12, e0174163 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
Q81E the mutant enzyme shows higher phosphorylating activity with 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone as compared to the wild type enzyme Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00068
-
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
0.0087
-
2'-deoxycytidine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
0.0113
-
2'-deoxycytidine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
0.0217
-
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
0.09
-
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
0.1017
-
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
0.4307
-
2'-deoxyguanosine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
1.612
-
2'-deoxyguanosine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a 2'-deoxyribonucleoside Drosophila melanogaster
-
ADP + a 2'-deoxyribonucleoside 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9XZT6
-
-

Purification (Commentary)

Purification (Comment) Organism
chelating Sepharose column chromatography and Superdex S200 gel filtration Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxycytidine
-
Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
?
ATP + 2'-deoxyguanosine
-
Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
?
ATP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one
-
Drosophila melanogaster ADP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one 5'-phosphate
-
?
ATP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone
-
Drosophila melanogaster ADP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone 5'-phosphate
-
?
ATP + a 2'-deoxyribonucleoside
-
Drosophila melanogaster ADP + a 2'-deoxyribonucleoside 5'-phosphate
-
?

Synonyms

Synonyms Comment Organism
dNK
-
Drosophila melanogaster

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00583
-
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
0.067
-
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
0.728
-
2'-deoxyguanosine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
0.817
-
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
1.283
-
2'-deoxyguanosine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
1.398
-
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
1.433
-
2'-deoxycytidine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
2.1
-
2'-deoxycytidine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0583
-
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
0.45
-
2'-deoxyguanosine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
3
-
2'-deoxyguanosine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
9
-
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
65
-
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
98.3
-
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster
165
-
2'-deoxycytidine wild type enzyme, at pH 7.6 and 37°C Drosophila melanogaster
183.3
-
2'-deoxycytidine mutant enzyme Q81E, at pH 7.6 and 37°C Drosophila melanogaster