Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
Q81E | the mutant enzyme shows higher phosphorylating activity with 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone as compared to the wild type enzyme | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00068 | - |
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.0087 | - |
2'-deoxycytidine | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.0113 | - |
2'-deoxycytidine | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.0217 | - |
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.09 | - |
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.1017 | - |
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.4307 | - |
2'-deoxyguanosine | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
1.612 | - |
2'-deoxyguanosine | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a 2'-deoxyribonucleoside | Drosophila melanogaster | - |
ADP + a 2'-deoxyribonucleoside 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9XZT6 | - |
- |
Purification (Comment) | Organism |
---|---|
chelating Sepharose column chromatography and Superdex S200 gel filtration | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one | - |
Drosophila melanogaster | ADP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one 5'-phosphate | - |
? | |
ATP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone | - |
Drosophila melanogaster | ADP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone 5'-phosphate | - |
? | |
ATP + a 2'-deoxyribonucleoside | - |
Drosophila melanogaster | ADP + a 2'-deoxyribonucleoside 5'-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dNK | - |
Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00583 | - |
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.067 | - |
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.728 | - |
2'-deoxyguanosine | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.817 | - |
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
1.283 | - |
2'-deoxyguanosine | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
1.398 | - |
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
1.433 | - |
2'-deoxycytidine | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
2.1 | - |
2'-deoxycytidine | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0583 | - |
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
0.45 | - |
2'-deoxyguanosine | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
3 | - |
2'-deoxyguanosine | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
9 | - |
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
65 | - |
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
98.3 | - |
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster | |
165 | - |
2'-deoxycytidine | wild type enzyme, at pH 7.6 and 37°C | Drosophila melanogaster | |
183.3 | - |
2'-deoxycytidine | mutant enzyme Q81E, at pH 7.6 and 37°C | Drosophila melanogaster |