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Literature summary for 2.7.1.145 extracted from

  • Jiang, H.; Zhao, L.; Dong, X.; He, A.; Zheng, C.; Johansson, M.; Karlsson, A.; Zheng, X.
    Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria (2015), Oncol. Rep., 34, 1487-1493.
    View publication on PubMed

Application

Application Comment Organism
medicine possible use of this enzyme as a suicide gene in vitro and in vivo Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression of Drosophila melanogaster enzyme Dm-dNK increases the sensitivity of cancer cells to several cytotoxic nucleoside analogues, recombinant expression of GFP-tagged mutant and wild-type enzymes in TK-1-deficient osteosarcoma cells. dThd kinase activities increased about 40fold in the cells expressing the nuclear dNK-GFP and about 35fold in the cells expressing the mitochondrial mito-dNK-GFP, compared with the untransduced parent cells Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion Dm-dNK localizes to the nucleus through a mitochondrial localization signal Drosophila melanogaster 5739
-
nucleus Dm-dNK localizes to the nucleus through a C-terminal nuclear localization signal Drosophila melanogaster 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxyribonucleoside Drosophila melanogaster
-
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9XZT6 gene dnk
-

Source Tissue

Source Tissue Comment Organism Textmining
carcinoma cell
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxyribonucleoside
-
Drosophila melanogaster ADP + 2'-deoxyribonucleoside 5'-phosphate
-
?
ATP + 2'-deoxythymidine
-
Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
?
additional information Dm-dNK phosphorylates a broad range of substrates including analogues of both purine and pyrimidine nucleosides, and exhibits a higher activity than other nucleoside kinases Drosophila melanogaster ?
-
?

Synonyms

Synonyms Comment Organism
Dm-dNK
-
Drosophila melanogaster
multi-substrate deoxyribonucleoside kinase
-
Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Drosophila melanogaster

General Information

General Information Comment Organism
physiological function no differences between mutant cytosolic Dm-dNK, mitochondrial Dm-dNK, and wild-type nuclear Dm-dNK in regards to enzyme activity, cellular sensitivity to nucleoside analogues, or bystander cell killing. Tan IIA may influence the bystander effect of cancer cells expressing Dm-dNK by regulating the expression of Cx43 and Cx26. Tanshinone (Tan) IIA is a fat-soluble and pharmacologically active ingredient of Danshen, a traditional Chinese medicine used in the treatment of cardiovascular diseases. It is isolated from the rhizome of a Chinese herb Salvia miltiorrhiza. Tan IIA treatment of osteosarcoma cells results in the upregulation of Cx43 and Cx26 proteins Drosophila melanogaster