Application | Comment | Organism |
---|---|---|
medicine | possible use of this enzyme as a suicide gene in vitro and in vivo | Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
heterologous expression of Drosophila melanogaster enzyme Dm-dNK increases the sensitivity of cancer cells to several cytotoxic nucleoside analogues, recombinant expression of GFP-tagged mutant and wild-type enzymes in TK-1-deficient osteosarcoma cells. dThd kinase activities increased about 40fold in the cells expressing the nuclear dNK-GFP and about 35fold in the cells expressing the mitochondrial mito-dNK-GFP, compared with the untransduced parent cells | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | Dm-dNK localizes to the nucleus through a mitochondrial localization signal | Drosophila melanogaster | 5739 | - |
nucleus | Dm-dNK localizes to the nucleus through a C-terminal nuclear localization signal | Drosophila melanogaster | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyribonucleoside | Drosophila melanogaster | - |
ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9XZT6 | gene dnk | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyribonucleoside | - |
Drosophila melanogaster | ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
additional information | Dm-dNK phosphorylates a broad range of substrates including analogues of both purine and pyrimidine nucleosides, and exhibits a higher activity than other nucleoside kinases | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dm-dNK | - |
Drosophila melanogaster |
multi-substrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
physiological function | no differences between mutant cytosolic Dm-dNK, mitochondrial Dm-dNK, and wild-type nuclear Dm-dNK in regards to enzyme activity, cellular sensitivity to nucleoside analogues, or bystander cell killing. Tan IIA may influence the bystander effect of cancer cells expressing Dm-dNK by regulating the expression of Cx43 and Cx26. Tanshinone (Tan) IIA is a fat-soluble and pharmacologically active ingredient of Danshen, a traditional Chinese medicine used in the treatment of cardiovascular diseases. It is isolated from the rhizome of a Chinese herb Salvia miltiorrhiza. Tan IIA treatment of osteosarcoma cells results in the upregulation of Cx43 and Cx26 proteins | Drosophila melanogaster |