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Literature summary for 2.7.1.145 extracted from

  • Gerth, M.L.; Lutz, S.
    Non-homologous recombination of deoxyribonucleoside kinases from human and Drosophila melanogaster yields human-like enzymes with novel activities (2007), J. Mol. Biol., 370, 742-751.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
additional information construction of libraries of hybrid enzymes by non-homologous recombination of the pyrimidinespecific human thymidine kinase 2 and the broad-specificity dNK from Drosophila melanogaster. Identification of chimeras that phosphorylate nucleoside analogs with higher activity than either parental enzyme, and that possess new activity towards the anti-HIV prodrug 2',3'-didehydro-3'-deoxythymidine Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km-values of chimeric enzymes Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-didehydro-3'-deoxythymidine + ATP
-
 Drosophila melanogaster 2',3'-didehydro-3'-deoxythymidine 5'-phosphate + ADP
-
 ?
2',3'-dideoxycytidine + ATP
-
 Drosophila melanogaster 2',3'-dideoxycytidine 5'-phosphate + ADP
-
 ?
2'-deoxyadenosine + ATP
-
 Drosophila melanogaster 2'-deoxyadenosine 5'-phosphate + ADP
-
 ?
2'-deoxycytidine + ATP
-
 Drosophila melanogaster 2'-deoxycytidine 5'-phosphate + ADP
-
 ?
2'-deoxyguanosine + ATP
-
 Drosophila melanogaster 2'-deoxyguanosine 5'-phosphate + ADP
-
 ?
ATP + 2'-deoxythymidine
-
 Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information turnover-numbers of chimeric enzymes Drosophila melanogaster