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Literature summary for 2.7.1.145 extracted from

  • Solaroli, N.; Bjerke, M.; Johansson, M.; Karlsson, A.
    Investigation of the substrate recognition of Drosophila melanogaster nucleoside kinase by site directed mutagenesis (2004), Nucleosides Nucleotides Nucleic Acids, 23, 1527-1529.
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme can be used as a suicide gene in combined gene/chemotherapy treatment of cancer Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes as glutathione-S-transferase fusion proteins in Escherichia coli Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
M88R site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme Drosophila melanogaster
V84A/M88R site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme Drosophila melanogaster
V84A/M88R/A110D site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
2'-deoxythymidine recombinant wild-type enzyme, pH 7.6, 37°C Drosophila melanogaster
0.79
-
2'-deoxythymidine recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C Drosophila melanogaster
0.92
-
ganciclovir recombinant mutant M88R, pH 7.6, 37°C Drosophila melanogaster
0.93
-
2'-deoxythymidine recombinant mutant M88R, pH 7.6, 37°C Drosophila melanogaster
1.2
-
ganciclovir recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C Drosophila melanogaster
1.75
-
ganciclovir recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C Drosophila melanogaster
2.68
-
ganciclovir recombinant mutant V84A/M88R, pH 7.6, 37°C Drosophila melanogaster
3.3
-
2'-deoxythymidine recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C Drosophila melanogaster
5.02
-
2'-deoxythymidine recombinant mutant V84A/M88R, pH 7.6, 37°C Drosophila melanogaster
5.06
-
ganciclovir recombinant mutant V84A/M88R/A110D, pH 7.6, 37°C Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes as glutathione-S-transferase fusion proteins from Escherichia coli by glutathione affinity chromatography Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-beta-D-arabinofuranosylguanine poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R Drosophila melanogaster ADP + 1-beta-D-arabinofuranosylguanine 5'-phosphate
-
?
ATP + 2',2'-difluorodeoxycytidine best substrate for the N-terminally truncated mutant M88R Drosophila melanogaster ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
?
ATP + 2',2'-difluorodeoxyguanosine poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R Drosophila melanogaster ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate
-
?
ATP + 2'-deoxyadenosine best substrate for the N-terminally truncated mutant V84A/M88R Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
?
ATP + 2'-deoxyguanosine poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
?
ATP + 2'-deoxythymidine best substrate for the wild-type enzyme Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
?
ATP + 9-beta-D-arabinofuranosyladenine poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R Drosophila melanogaster ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
?
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
-
Drosophila melanogaster ADP + E-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
?
ATP + ganciclovir poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R Drosophila melanogaster ADP + ganciclovir phosphate
-
?
additional information broad substrate specificity Drosophila melanogaster ?
-
?

Synonyms

Synonyms Comment Organism
deoxyribonucleoside kinase
-
Drosophila melanogaster
dNK
-
Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Drosophila melanogaster