Application | Comment | Organism |
---|---|---|
medicine | the enzyme can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes as glutathione-S-transferase fusion proteins in Escherichia coli | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
M88R | site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
V84A/M88R | site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
V84A/M88R/A110D | site-directed mutagenesis of the full length enzyme and of the DELTA20 N-terminally truncated enzyme, highly increased Km for acceptor substrates, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
2'-deoxythymidine | recombinant wild-type enzyme, pH 7.6, 37°C | Drosophila melanogaster | |
0.79 | - |
2'-deoxythymidine | recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
0.92 | - |
ganciclovir | recombinant mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
0.93 | - |
2'-deoxythymidine | recombinant mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
1.2 | - |
ganciclovir | recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C | Drosophila melanogaster | |
1.75 | - |
ganciclovir | recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
2.68 | - |
ganciclovir | recombinant mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
3.3 | - |
2'-deoxythymidine | recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
5.02 | - |
2'-deoxythymidine | recombinant mutant V84A/M88R, pH 7.6, 37°C | Drosophila melanogaster | |
5.06 | - |
ganciclovir | recombinant mutant V84A/M88R/A110D, pH 7.6, 37°C | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes as glutathione-S-transferase fusion proteins from Escherichia coli by glutathione affinity chromatography | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-arabinofuranosylguanine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 1-beta-D-arabinofuranosylguanine 5'-phosphate | - |
? | |
ATP + 2',2'-difluorodeoxycytidine | best substrate for the N-terminally truncated mutant M88R | Drosophila melanogaster | ADP + 2',2'-difluorodeoxycytidine 5'-phosphate | - |
? | |
ATP + 2',2'-difluorodeoxyguanosine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | best substrate for the N-terminally truncated mutant V84A/M88R | Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | best substrate for the wild-type enzyme | Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 9-beta-D-arabinofuranosyladenine | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate | - |
? | |
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine | - |
Drosophila melanogaster | ADP + E-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + ganciclovir | poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R | Drosophila melanogaster | ADP + ganciclovir phosphate | - |
? | |
additional information | broad substrate specificity | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyribonucleoside kinase | - |
Drosophila melanogaster |
dNK | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |