Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli as GST-fusion proteins | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
F114A | site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
F114Y | site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
I29H | site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
I29H/F114Y | site-directed mutagenesis, active site mutant, inactive mutant | Drosophila melanogaster |
N28P | site-directed mutagenesis, active site mutant, inactive mutant | Drosophila melanogaster |
N28P/I29H | site-directed mutagenesis, active site mutant, inactive mutant | Drosophila melanogaster |
N28P/I29H/F114Y | site-directed mutagenesis, active site mutant, inactive mutant | Drosophila melanogaster |
Q81D | site-directed mutagenesis, active site mutant, inactive mutant | Drosophila melanogaster |
Q81N | site-directed mutagenesis, active site mutant, altered substrate specificity compared to the wild-type enzyme | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
2'-deoxythymidine | pH 7.6, 37°C, recombinant wild-type enzyme | Drosophila melanogaster | |
0.0031 | - |
2'-deoxythymidine | pH 7.6, 37°C, recombinant mutant F114A | Drosophila melanogaster | |
0.0047 | - |
2'-deoxythymidine | pH 7.6, 37°C, recombinant mutant Q81N | Drosophila melanogaster | |
0.022 | - |
2'-deoxythymidine | pH 7.6, 37°C, recombinant mutant F114Y | Drosophila melanogaster | |
0.1 | - |
2'-deoxythymidine | above, pH 7.6, 37°C, recombinant mutants I29H and Q81D | Drosophila melanogaster | |
0.373 | - |
2'-deoxyadenosine | pH 7.6, 37°C, recombinant wild-type enzyme | Drosophila melanogaster | |
0.625 | - |
2'-deoxyadenosine | pH 7.6, 37°C, recombinant mutant Q81N | Drosophila melanogaster | |
0.985 | - |
2'-deoxyguanosine | pH 7.6, 37°C, recombinant mutant Q81N | Drosophila melanogaster | |
2 | - |
2'-deoxyguanosine | pH 7.6, 37°C, recombinant wild-type enzyme | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli by glutathione affinity chromatography | Drosophila melanogaster |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificities of wild-type and mutant enzymes | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-arabinofuranosylcytosine | - |
Drosophila melanogaster | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
ATP + 2',2'-difluorodeoxycytidine | - |
Drosophila melanogaster | ADP + 2',2'-difluorodeoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | poor substrate for the wild-type enzyme | Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2-chlorodeoxyadenosine | - |
Drosophila melanogaster | ADP + 2-chlorodeoxyadenosine 5'-phosphate | - |
? | |
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine | - |
Drosophila melanogaster | ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate | - |
? | |
additional information | substrate specificities of wild-type and mutant enzymes, overview, no or poor activity with ganciclovir | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dNK | - |
Drosophila melanogaster |
multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |