Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
M88R | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
M88R/A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
V84A | site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
V84A/M88R/A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
V84M | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
V84S | site-directed mutagenesis, altered substrate specificity and kinetics, and reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
V84S/M88R/A110D | site-directed mutagenesis, altered substrate specificity and kinetics, and highly reduced activity compared to the wild-type enzyme | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
thymidine | recombinant wild-type enzyme | Drosophila melanogaster | |
0.0023 | - |
2'-deoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
0.0025 | - |
2'-deoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
0.0113 | - |
thymidine | recombinant mutant V84A | Drosophila melanogaster | |
0.0129 | - |
2'-deoxycytidine | recombinant mutant V84S | Drosophila melanogaster | |
0.0247 | - |
thymidine | recombinant mutant V84S | Drosophila melanogaster | |
0.04 | - |
2'-deoxyguanosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
0.064 | - |
thymidine | recombinant mutant A110D | Drosophila melanogaster | |
0.091 | - |
2'-deoxycytidine | recombinant mutant A110D | Drosophila melanogaster | |
0.147 | - |
2'-deoxyguanosine | recombinant mutant V84A | Drosophila melanogaster | |
0.15 | - |
2'-deoxyadenosine | recombinant mutant V84S | Drosophila melanogaster | |
0.153 | - |
2'-deoxyadenosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
0.159 | - |
2'-deoxyguanosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
0.225 | - |
2'-deoxyadenosine | recombinant wild-type enzyme | Drosophila melanogaster | |
0.237 | - |
thymidine | recombinant mutant M88R | Drosophila melanogaster | |
0.239 | - |
2'-deoxyadenosine | recombinant mutant A110D | Drosophila melanogaster | |
0.257 | - |
2'-deoxyguanosine | recombinant mutant A110D | Drosophila melanogaster | |
0.292 | - |
Dideoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
0.36 | - |
2'-deoxyadenosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
0.375 | - |
2'-deoxyguanosine | recombinant mutant V84S | Drosophila melanogaster | |
0.388 | - |
2'-deoxyadenosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
0.549 | - |
2'-deoxyguanosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
0.584 | - |
2'-deoxycytidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
0.627 | - |
2'-deoxycytidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
0.645 | - |
2'-deoxyguanosine | recombinant mutant M88R | Drosophila melanogaster | |
0.665 | - |
2'-deoxyguanosine | recombinant wild-type enzyme | Drosophila melanogaster | |
0.674 | - |
2'-deoxycytidine | recombinant mutant V84M | Drosophila melanogaster | |
0.7 | - |
2'-deoxyadenosine | recombinant mutant V84A | Drosophila melanogaster | |
0.829 | - |
thymidine | recombinant mutant V84M | Drosophila melanogaster | |
0.852 | - |
2'-deoxyadenosine | recombinant mutant V84M | Drosophila melanogaster | |
1.039 | - |
2'-deoxycytidine | recombinant mutant M88R | Drosophila melanogaster | |
1.124 | - |
Dideoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
1.512 | - |
thymidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
1.911 | - |
2'-deoxyguanosine | recombinant mutant V84M | Drosophila melanogaster | |
2.088 | - |
2'-deoxyadenosine | recombinant mutant M88R | Drosophila melanogaster | |
2.162 | - |
2'-deoxycytidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
2.425 | - |
thymidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
6.56 | - |
thymidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | enzyme evolution by gene duplication of a progenitor kinase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His8-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography | Drosophila melanogaster |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic efficiencies of wild-type and mutant enzymes, overview | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | wild-type enzyme: second-best substrate | Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + dideoxycytidine | wild-type enzyme: low activity | Drosophila melanogaster | ADP + dideoxycytidine 5'-phosphate | - |
? | |
ATP + thymidine | wild-type enzyme: preferred substrate | Drosophila melanogaster | ADP + thymidine 5'-phosphate | - |
? | |
additional information | enzyme evolution by gene duplication of a progenitor kinase | Drosophila melanogaster | ? | - |
? | |
additional information | amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview | Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dNK | - |
Drosophila melanogaster |
multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
thymidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
0.037 | - |
thymidine | recombinant mutant V84M | Drosophila melanogaster | |
0.073 | - |
2'-deoxyguanosine | recombinant mutant A110D | Drosophila melanogaster | |
0.084 | - |
2'-deoxyadenosine | recombinant mutant A110D | Drosophila melanogaster | |
0.11 | - |
thymidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
0.13 | - |
thymidine | recombinant mutant M88R | Drosophila melanogaster | |
0.22 | - |
2'-deoxyguanosine | recombinant mutant V84M | Drosophila melanogaster | |
0.23 | - |
thymidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
0.27 | - |
2'-deoxycytidine | recombinant mutant A110D | Drosophila melanogaster | |
0.35 | - |
thymidine | recombinant mutant A110D | Drosophila melanogaster | |
0.64 | - |
2'-deoxyguanosine | recombinant mutant V84S | Drosophila melanogaster | |
0.83 | - |
thymidine | recombinant mutant V84S | Drosophila melanogaster | |
0.83 | - |
2'-deoxycytidine | recombinant mutant V84S | Drosophila melanogaster | |
0.93 | - |
Dideoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
1.1 | - |
2'-deoxyadenosine | recombinant mutant V84S | Drosophila melanogaster | |
1.36 | - |
2'-deoxyadenosine | recombinant mutant V84M | Drosophila melanogaster | |
1.62 | - |
2'-deoxycytidine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
1.73 | - |
2'-deoxyguanosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
1.8 | - |
2'-deoxycytidine | recombinant mutant V84M | Drosophila melanogaster | |
1.88 | - |
2'-deoxyadenosine | recombinant mutant M88R | Drosophila melanogaster | |
1.94 | - |
2'-deoxycytidine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
2.7 | - |
2'-deoxyguanosine | recombinant mutant M88R | Drosophila melanogaster | |
2.8 | - |
2'-deoxyguanosine | recombinant mutant V84A | Drosophila melanogaster | |
2.97 | - |
thymidine | recombinant mutant V84A | Drosophila melanogaster | |
3.2 | - |
2'-deoxyadenosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
3.5 | - |
2'-deoxycytidine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
3.5 | - |
2'-deoxyadenosine | recombinant mutant V84A/M88R/A110D | Drosophila melanogaster | |
3.7 | - |
2'-deoxyguanosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
3.7 | - |
2'-deoxyadenosine | recombinant mutant V84A | Drosophila melanogaster | |
4.2 | - |
Dideoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
4.4 | - |
2'-deoxycytidine | recombinant mutant V84A | Drosophila melanogaster | |
4.6 | - |
2'-deoxyguanosine | recombinant mutant V84S/M88R/A110D | Drosophila melanogaster | |
6.9 | - |
2'-deoxyadenosine | recombinant mutant M88R/A110D | Drosophila melanogaster | |
7.5 | - |
2'-deoxycytidine | recombinant mutant M88R | Drosophila melanogaster | |
14.2 | - |
thymidine | recombinant wild-type enzyme | Drosophila melanogaster | |
15.1 | - |
2'-deoxyguanosine | recombinant wild-type enzyme | Drosophila melanogaster | |
16.5 | - |
2'-deoxycytidine | recombinant wild-type enzyme | Drosophila melanogaster | |
20.6 | - |
2'-deoxyadenosine | recombinant wild-type enzyme | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |