Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.145 extracted from

  • Eriksson, S.; Munch-Petersen, B.; Johansson, K.; Eklund, H.
    Structure and function of cellular deoxyribonucleoside kinases (2002), Cell. Mol. Life Sci., 59, 1327-1346.
    View publication on PubMed
Search for more literature for 2.7.1.145

Application

Application Comment Organism
medicine enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer Bombyx mori
medicine enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer Drosophila melanogaster
synthesis enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes Drosophila melanogaster
synthesis enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used forDNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes Bombyx mori

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895 Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
N45D/N64D site-directed mutagenesis, mutant shows increased drug sensitivity and decreased inhibition by dTTP compared to the wild-type enzyme Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
dTTP competitive versus ATP, feedback inhibition Bombyx mori
dTTP competitive versus ATP, feedback inhibition Drosophila melanogaster
additional information no inhibition by dNTPs other than dTTP Bombyx mori
additional information no inhibition by dNTPs other than dTTP Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics Bombyx mori
additional information
-
 additional information straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Bombyx mori
Mg2+ dependent on Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 2'-deoxyadenosine Drosophila melanogaster
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine Drosophila melanogaster
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine Drosophila melanogaster
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
ATP + 2'-deoxythymidine Drosophila melanogaster
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-
Drosophila melanogaster Q9XZT6 dNK is the only deoxyriconucleoside kinase in the fruitfly
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate positive cooperativity mechanism, substrate and nucleotide interactions and binding sites, overview Bombyx mori
a
ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex, substrate interactions Drosophila melanogaster
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-beta-D-arabinofuranosylcytosine
-
 Drosophila melanogaster ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
ATP + 1-beta-D-arabinofuranosylthymine
-
 Drosophila melanogaster ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
 ?
ATP + 2'-deoxyadenosine
-
 Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 2'-deoxycytidine
-
 Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
ATP + 2'-deoxyguanosine
-
 Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
ATP + 2'-deoxythymidine
-
 Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
ATP + 2-chloro-2'-deoxyadenosine
-
 Drosophila melanogaster ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
ATP + 5-fluoro-2'-deoxyuridine
-
 Drosophila melanogaster ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
-
 Drosophila melanogaster ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
-
 ?
additional information broad substrate specificity Drosophila melanogaster ?
-
 ?
additional information substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides Bombyx mori ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Bombyx mori
dimer
-
 Drosophila melanogaster
More ternary and quarternary structure analysis, overview Bombyx mori
More ternary and quarternary structure analysis, overview Drosophila melanogaster

Synonyms

Synonyms Comment Organism
dNK
-
 Bombyx mori
dNK
-
 Drosophila melanogaster
More the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes Bombyx mori
More the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes Drosophila melanogaster
multisubstrate deoxyribonucleoside kinase
-
 Bombyx mori
multisubstrate deoxyribonucleoside kinase
-
 Drosophila melanogaster
nucleoside triphosphate: deoxyribonucleoside 5'-phosphotransferase
-
 Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Drosophila melanogaster