Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil | IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
(E)-5-(2-bromovinyl)-2'-deoxyuridine | IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil | IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
1-beta-D-arabinofuranosylcytosine | IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
1-beta-D-arabinofuranosylthymidine | IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
1-beta-D-arabinofuranosyluracil | IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
2',2'-difluorodeoxycytidine | IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
2',3'-dehydro-3'-deoxythymidine | IC50 for reaction with thymidine is greater than 1000 mM, IC50 for reaction with 2'-deoxycytidine is 0.805 mM | Drosophila melanogaster | |
2',3'-dideoxycytidine | IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
2',3'-dideoxythymidine | IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
2'-deoxy-3'-thiacytidine | IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
2'-deoxycytidine | competitive with thymidine | Drosophila melanogaster | |
2'-deoxyuridine | reaction with thymidine and 2'-deoxycytidine | Drosophila melanogaster | |
2-chloro-2'-deoxyadenosine | IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
3'-azido-2',3'-dideoxythymidine | IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
5-fluoro-2'-deoxyuridine | IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | |
thymidine | competitive with 2'-deoxycytidine | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
thymidine | pH 8, 37°C | Drosophila melanogaster | |
0.0026 | - |
2'-deoxycytidine | pH 8, 37°C | Drosophila melanogaster | |
0.373 | - |
2'-deoxyadenosine | pH 8, 37°C | Drosophila melanogaster | |
2 | - |
2'-deoxyguanosine | pH 8, 37°C | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
1 * 29000, calculation from nucleotide sequence | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9XZT6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxycytidine + ATP | - |
Drosophila melanogaster | 2'-deoxycytidine 5'-phosphate + ADP | - |
? | |
additional information | the recombinant enzyme preferentially phosphorylates the pyrimidine nucleosides, but phosphorylation of the purine nucleosidesis also efficiently catalyzed | Drosophila melanogaster | ? | - |
? | |
additional information | no activity with TMP | Drosophila melanogaster | ? | - |
? | |
thymidine + ATP | - |
Drosophila melanogaster | thymidine 5'-phosphate + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 29000, calculation from nucleotide sequence | Drosophila melanogaster |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00257 | - |
IC50: 0.00257 mM for reaction with thymidine and 0.00194 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | (E)-5-(2-bromovinyl)-2'-deoxyuridine | |
0.0163 | - |
IC50: 0.0163 mM for reaction with thymidine and 0.0154 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)-5-iodouracil | |
0.0258 | - |
IC50: 0.0258 mM for reaction with thymidine and 0.02235 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 5-fluoro-2'-deoxyuridine | |
0.0277 | - |
IC50: 0.0277 mM for reaction with thymidine and 0.0158 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | (E)-5-(2-bromovinyl)-1-beta-D-arabinofuranosyluracil | |
0.0419 | - |
IC50: 0.0419 mM for reaction with thymidine and 0.0296 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 3'-azido-2',3'-dideoxythymidine | |
0.0534 | - |
IC50: 0.0534 mM for reaction with thymidine and 0.089 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 1-beta-D-arabinofuranosylcytosine | |
0.057 | - |
IC50: 0.057 mM for reaction with thymidine and 0.058 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 1-beta-D-arabinofuranosyluracil | |
0.0648 | - |
IC50: 0.0648 mM for reaction with thymidine and 0.0348 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 1-beta-D-arabinofuranosylthymidine | |
0.075 | - |
IC50: 0.075 mM for reaction with thymidine and 0.0703 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 2',3'-dideoxythymidine | |
0.123 | - |
IC50: 0.123 mM for reaction with thymidine and 0.12 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 2-chloro-2'-deoxyadenosine | |
0.145 | - |
IC50: 0.145 mM for reaction with thymidine and 0.102 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 2',2'-difluorodeoxycytidine | |
0.74 | - |
IC50: 0.74 mM for reaction with thymidine and 0.868 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 2'-deoxy-3'-thiacytidine | |
0.76 | - |
IC50: 0.76 mM for reaction with thymidine and 0.838 mM for reaction with 2'-deoxycytidine | Drosophila melanogaster | 2',3'-dideoxycytidine | |
0.805 | - |
IC50 for reaction with 2'-deoxycytidine is 0.805 mM | Drosophila melanogaster | 2',3'-dehydro-3'-deoxythymidine | |
1 | - |
IC50 for reaction with thymidine is greater than 1000 microM | Drosophila melanogaster | 2',3'-dehydro-3'-deoxythymidine |